SLC9A2 antibody (Intracellular)

Catalog No. ABIN7043757

Product Details anti-SLC9A2 Antibody

Target: SLC9A2 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 2 (SLC9A2))

Binding Specificity: AA 797-812, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC9A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 2 (NHE-2)

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 12,16 amino acid residues identical

Characteristics: Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody (ABIN7043757, ABIN7044867 and ABIN7044868)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NHE-2 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RASEPGNRKGRLGNEK, corresponding to amino acid residues 797-812 of rat NHE-2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:50

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-NX002

Blocking Peptide: BLP-NX002

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC9A2

Target: SLC9A2 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 2 (SLC9A2))

Alternative Name: SLC9A2 (SLC9A2 Products)

Background: Sodium/hydrogen exchanger 2, SLC9A2,In order to function in optimal conditions, cells must maintain a close to neutral intracellular pH . They have adopted various mechanisms in order to do so, one of which is via Na+/H+ exchangers (NHEs). Genes belonging to this group are expressed along a very broad range of organisms and are essential for protecting cells against intracellular acidification1.To date, nine genes have been identified in mammals, NHE1-9. These membrane proteins have 10-12 transmembrane domains depending on whether a splice variant is expressed and an intracellular N-terminal. The C-terminal domain can be either intracellular or extracellular, also depending whether a splice variant of the protein is involved. The C-terminal part of the protein also undergoes posttranslational modification such as phosphorylation2. Both NHE-1 and NHE-2 have an extracellular loop which is glycosylated1,3,4.Under physiological conditions, the Na+/H+ exchanger mediates the exchange of one extracellular Na+ ion for one intracellular proton, thereby keeping the overall charge neutral1. The extracellular binding site of Na+ is not selective as it can also bind Li+ and H+ 1,5,6. K+ ions inhibit NHE-1 but have no effect on NHE-27. The activation of NHE-1 and NHE-2 is sensitive to intracellular acidic pH . Under physiological conditions, both exchangers are not active and upon a drop of intracellular pH , they are rapidly activated1,5,8.NHE-2 is detected in the intestine, kidney and parietal cells2,9-11. It is also detected in skeletal muscle and testis2,12.

Alternative names: Na+/H+ Exchanger 2 (NHE-2), Sodium/hydrogen exchanger 2, SLC9A2

Gene ID: 24783

NCBI Accession: NM_003048

UniProt: P48763

Pathways: Proton Transport