SLC9A5 antibody (Intracellular)

Catalog No. ABIN7043759

Product Details anti-SLC9A5 Antibody

Target: SLC9A5 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 5 (SLC9A5))

Binding Specificity: AA 759-772, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC9A5 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 5 (NHE-5)

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Na+/H+ Exchanger 5 (NHE-5) Antibody is directed against an epitope of rat NHE-5. Anti-Na+/H+ Exchanger 5 (NHE-5) Antibody (ABIN7043759, ABIN7044869 and ABIN7044870)) can be used in western blot analysis. It has been designed to recognize NHE-5 from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CAEKELPWKSGQGD, corresponding to amino acid residues 759-772 of rat NHE-5

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-NX005

Blocking Peptide: BLP-NX005

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC9A5

Target: SLC9A5 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 5 (SLC9A5))

Alternative Name: SLC9A5 (SLC9A5 Products)

Background: Sodium/hydrogen exchanger 5, SLC9A5,Intracellular pH regulation in the brain is extremely important under both physiological and physiopathological conditions. Changes in intracellular pH often result in altered neuronal excitability. In mammalian cells, intracellular pH against acidosis is generally regulated by ion transporters located in the plasma membrane called sodium-hydrogen exchangers (Na+/H+ exchanger, NHE)1. Several studies have suggested that Na+/H+ exchange is the predominant pH -regulatory mode in isolated nerve terminals. Na+/H+ exchanger also has a role in the regulation of synaptic transmission at glutamatergic, GABAergic, and dopaminergic synapses. In addition, NHE transporters also function in a coupled mode to mediate recovery from decreased cell volume1. The NHE family belongs to the Slc9 gene family of Na+ coupled transport proteins and comprises nine mammalian isoforms of Na+/H+ exchangers that are localized to the plasma membrane. NHE1-9 are encoded by the SLC9A1-SLC9A9 genes, respectively. The structure of SLC9 family members includes a short N-terminus and an extensive C-terminus that plays a regulatory role. NHE-5 exchanger corrects intracellular acidosis by exporting H+ in exchange for extracellular Na+, thereby tending to raise intracellular pH . Several studies have suggested that NHE5 plays a critical role in the synaptic pH regulation during the firing of action potentials1. It is predominantly detected in the brain with high expression in multiple regions such as the hippocampus and cerebral cortex3. NHE5 knockdown or overexpression of a dominant-negative mutation of NHE5 in cultured hippocampal neurons causes dendritic spine overgrowth. Therefore it has been suggested that NHE5 acts as a negative regulator of activity-dependent spine growth by regulating pH -sensitive synaptic proteins2,3.

Alternative names: Na+/H+ Exchanger 5 (NHE-5), Sodium/hydrogen exchanger 5, SLC9A5

Gene ID: 192215

NCBI Accession: NM_001323971

UniProt: Q9Z0X2

Pathways: Proton Transport