TRPV1 antibody (Cys616Ser-Mutant, Extracellular) (Atto 488)

Catalog No. ABIN7043839

Product Details anti-TRPV1 Antibody (Atto 488)

Target: TRPV1 (Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1))

Binding Specificity: AA 605-619, Cys616Ser-Mutant, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRPV1 antibody is conjugated to Atto 488

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Live Cell Imaging (LCI), Immunochromatography (IC), Flow Cytometry (FACS)

Purpose: A Rabbit Polyclonal Antibody to Rat TRPV1 (VR1) Channel Conjugated to the Fluorescent Dye ATTO-488

Specificity: 3rd extracellular loop

Cross-Reactivity (Details): Recognizes TRPV1 from rat only.

Predicted Reactivity: Rat - 14 out of 15 amino acid residues identical Mouse - 12 out of 15 amino acid residues identicalWon't recognize TRPV1 from human samples

Characteristics: Anti-Rat TRPV1 (VR1) (extracellular) Antibody (ABIN7043838, ABIN7043986 and ABIN7043987) is a highly specific antibody directed against an extracellular epitope of the rat TRPV1 channel. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize TRPV1 from rat samples only. \nAnti-Rat-TRPV1 (VR1) (extracellular)-ATTO Fluor-488 Antibody (ABIN7043839) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Rat-TRPV1 (VR1) (extracellular)-ATTO Fluor-488 Antibody has been tested in immunohistochemistry and live cell imaging applications and is especially suited for experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)NSLPMESTPHK*SRGS, corresponding to amino acid residues 605-619 of rat TRPV1 with replacement of cysteine 616 (C616) with serine (*S)

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:50-1:60

Application Dilutions Western blot wb: N/A

Comment:

Cited Application: IHC

Negative Control: (ABIN7582041)

Blocking Peptide:

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TRPV1

Target: TRPV1 (Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1))

Alternative Name: TRPV1 (TRPV1 Products)

Background: Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1,TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1According to IUPHAR the TRP family comprises three main subfamilies on the basis of sequence homology, TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family, the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5TRPV1 channel has many activators, among them heat, protons, vanilloids like capsaicin, resiniferatoxin (RTX), and lipids. This channel is associated with tissue injury and inflammation.6,7 TRPV1 is expressed predominantly in nociceptors and in sensory neurons.Recent studies demonstrated involvement of TRPV1 in apoptosis where inhibition of the receptor prevented apoptosis.8,9

Alternative names: TRPV1 (VR1), Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1

Gene ID: 83810

NCBI Accession: NM_018727

UniProt: O35433

Pathways: Dicarboxylic Acid Transport