TRPV4 antibody (Intracellular)

Catalog No. ABIN7043850

Product Details anti-TRPV4 Antibody

Target: TRPV4 (Transient Receptor Potential Cation Channel, Subfamily V, Member 4 (TRPV4))

Binding Specificity: AA 853-871, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRPV4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunofluorescence (Cultured Cells) (IF (cc)), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to TRPV4 Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: human - 16,19 amino acid residues identical,Mouse - 17

Characteristics: Anti-TRPV4 Antibody (ABIN7043850, ABIN7043994 and ABIN7043995)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize TRPV4 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CDGHQQGYAPKWRAEDAPL, corresponding to amino acid residues 853-871 of rat TRPV4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|IHC|ICC|IFC

Negative Control: BLP-CC034

Blocking Peptide: BLP-CC034

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TRPV4

Target: TRPV4 (Transient Receptor Potential Cation Channel, Subfamily V, Member 4 (TRPV4))

Alternative Name: TRPV4 (TRPV4 Products)

Background: OTRPC4, VRL-2, VR-OAC, TRP12,TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology, TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family, the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland.6

Alternative names: TRPV4, OTRPC4, VRL-2, VR-OAC, TRP12

Gene ID: 66026

NCBI Accession: NM_021625

UniProt: Q9ERZ8

Pathways: Hormone Transport, Cell-Cell Junction Organization