TRPV5 antibody (Intracellular)

Catalog No. ABIN7043855

Product Details anti-TRPV5 Antibody

Target: TRPV5 (Transient Receptor Potential Cation Channel, Subfamily V, Member 5 (TRPV5))

Binding Specificity: AA 715-729, Intracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRPV5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to TRPV5 Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: rabbit - 13,Rat - 11,15 amino acid residues identical

Characteristics: Anti-TRPV5 Antibody is directed against an epitope of human TRPV5. Anti-TRPV5 Antibody (ABIN7043855, ABIN7043996 and ABIN7043997)) can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize TRPV5 from human, mouse and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GLNLSEGDGEEVYHF, corresponding to amino acid residues 715-729 of human TRPV5

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: ICC|IFC

Negative Control: BLP-CC035

Blocking Peptide: BLP-CC035

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TRPV5

Target: TRPV5 (Transient Receptor Potential Cation Channel, Subfamily V, Member 5 (TRPV5))

Alternative Name: TRPV5 (TRPV5 Products)

Background: Transient receptor potential cation channel subfamily V member 5, Calcium transport protein 2, CaT2, Epithelial calcium channel 1, ECaC1, OTRPC3,TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 According to IUPHAR, the TRP family is comprised of three main subfamilies on the basis of sequence homology, TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family, the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5TRPV5 (ECaC) and TRPV6 (CaT1) are unique members of the TRP family. Both are epithelial Ca2+ channels, form constitutively open channels, share high degree of homology (about 66 % ) with differences in the N- and C-terminus. TRPV6 and TRPV5 are expressed in a 1,25-dihydroxyvitamin D3 dependent manner. In contrast to other members of the TRP family, TRPV5 and TRPV6 are highly Ca2+ selective and are expressed in Ca2+ transporting epithelia where they are assumed to have an important role in Ca2+ reabsorption.6,7 TRPV5 is preferentially expressed in the kidney.

Alternative names: TRPV5, Transient receptor potential cation channel subfamily V member 5, Calcium transport protein 2, CaT2, Epithelial calcium channel 1, ECaC1, OTRPC3

Gene ID: 56302

NCBI Accession: NM_019841

UniProt: Q8NDW5