P2RX3 antibody (Intracellular)

Catalog No. ABIN7237745

Product Details anti-P2RX3 Antibody

Target: P2RX3 (Purinergic Receptor P2x, Ligand-Gated Ion Channel, 3 (P2RX3))

Binding Specificity: AA 383-397, Intracellular

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This P2RX3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Guinea Pig Polyclonal Antibody to P2X3 Receptor

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 13 out of 15 amino acid residues identical

Characteristics: Intracellular, C-terminus

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)VEKQSTDSGAYSIGH, corresponding to amino acid residues 383-397 of rat P2X3 receptor 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: BLP-PR016

Blocking Peptide: BLP-PR016

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for P2RX3

Target: P2RX3 (Purinergic Receptor P2x, Ligand-Gated Ion Channel, 3 (P2RX3))

Alternative Name: P2RX3 (P2RX3 Products)

Background: P2RX3, P2X purinoceptor 3, ATP Receptor,The P2X3 receptor belongs to the ligand-gated ion channel P2X receptor family, that consists of seven receptor subtypes named P2X1-P2X7 and is activated by extracellular ATP.1,2,3All P2X subunits, with the exception of P2X6, can assemble to form homomeric or heteromeric functional channels.4-5 The different P2X receptors show distinct expression patterns. P2X1-6 has been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system. The P2X3 receptor is highly expressed on nociceptive sensory neurons in dorsal root ganglia (DRG) as a homomer or as a heteromer (P2X3/P2X2). ATP released from damaged cells activates the P2X3 receptor to initiate nociceptive signals.6,7 Involvement of ATP in the mechanism of chronic pain has been also suggested.7,8 P2X3 receptor is now becoming a possible target for the development of pain therapeutics.

Alternative names: P2RX3, P2X purinoceptor 3, ATP Receptor

Gene ID: 81739

NCBI Accession: NM_002559

UniProt: P49654