eEF1A1 antibody (N-Term)

Catalog No. ABIN7540532

Supplier Product No.: 200-901-mp6

Product Details anti-eEF1A1 Antibody

Target: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Binding Specificity: N-Term

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Conjugate: This eEF1A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 200-901-mp6

Supplier: Rockland

Purpose: EEF1A1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human EEF1A1.

Purification: This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen: Anti-EEF1A1 antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to a N-terminal portion of human EEF1A1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:100

Western_Blot_Dilution: 5 μg/mL

Comment:

Anti-EEF1A1 Antibody has been tested in Western Blot and IHC.  Expect a band at ~48-50 kDa in western blot using appropriate tissues and lysates.  Positive control used: MCF-7 nuclear extract lysate in western blot and human kidney tissue in Immunohistochemistry.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.002 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.0

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for eEF1A1

Target: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Alternative Name: EEF1A1 (EEF1A1 Products)

Background: Chicken Anti-Eukaryotic Translation Elongation Factor 1 Alpha 1, Leukocyte Receptor Cluster (LRC) Member 7, Leukocyte Receptor Cluster Member 7, Eukaryotic Elongation Factor 1 A-1, Elongation Factor 1-Alpha 1, Elongation Factor Tu, EF-1-Alpha-1, EEF1A-1, EEF1A, LENG7, EF-Tu, EF1A, Eukaryotic Translation Elongation Factor 1 Alpha 1-Like 14, Glucocorticoid Receptor AF-1 Specific Elongation Factor, Translation Elongation Factor 1 Alpha 1-Like 14, Epididymis Secretory Sperm Binding Protein, Elongation Factor 1 Alpha Subunit, Prostate Tumor-Inducing Protein 1, Cervical Cancer Suppressor 3, CTCL Tumor Antigen, EF1a-Like Protein, HNGC:16303, GRAF-1EF, CCS-3, EE1A1, EEF-1, CCS3, PTI1,EEF1A1 (Eukaryotic Translation Elongation Factor 1 Alpha 1) protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66 % of patients with Felty syndrome. Anti-EEF1A1 is useful for researchers interested in Eukaryotic Translation Elongation Factor 1 Alpha-1-Like 14, Cervical Cancer, and the Innate Immune system.

Gene ID: 1915

NCBI Accession: NP_001393

UniProt: P68104