Integrin beta 3 antibody (ITGB3) (FITC)

Catalog No. ABIN810079

Product Details anti-Integrin beta 3 Antibody

Target:

Integrin beta 3 (ITGB3)

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 3 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)

Specificity: The mouse monoclonal antibody VIPL2 recognizes CD61, a 90-110 kDa transmembrane glycoprotein of integrin family, expressed on platelets, megacaryocytes, osteoclasts, endothelial cells and other cell types, including leucocytes and smooth muscle cells.
HLDA V., WS Code 5T-124

Cross-Reactivity (Details): Human, Non-Human Primates

Clone: VIPL2

Isotype: IgG1

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 4 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (0.4 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-Integrin beta 3 antibody (ABIN810079)

Barrett, Dai, Gamberg, Gallant, Grant: "Circulating CD14-CD36+ peripheral blood mononuclear cells constitutively produce interleukin-10." in: Journal of leukocyte biology, Vol. 82, Issue 1, pp. 152-60, 2007 (PubMed).

Mondal, Williams, Ali, Eilers, Agrawal: "The HIV-1 Tat protein selectively enhances CXCR4 and inhibits CCR5 expression in megakaryocytic K562 cells." in: Experimental biology and medicine (Maywood, N.J.), Vol. 230, Issue 9, pp. 631-44, 2005 (PubMed).

Williams, Mondal, Agrawal: "The HIV-1 Tat protein enhances megakaryocytic commitment of K562 cells by facilitating CREB transcription factor coactivation by CBP." in: Experimental biology and medicine (Maywood, N.J.), Vol. 230, Issue 11, pp. 872-84, 2005 (PubMed).

Roberts, Woods, Dale, Van Der Sluijs, Norman: "Protein kinase B/Akt acts via glycogen synthase kinase 3 to regulate recycling of alpha v beta 3 and alpha 5 beta 1 integrins." in: Molecular and cellular biology, Vol. 24, Issue 4, pp. 1505-15, 2004 (PubMed).

Ciarlet, Crawford, Cheng, Blutt, Rice, Bergelson, Estes: "VLA-2 (alpha2beta1) integrin promotes rotavirus entry into cells but is not necessary for rotavirus attachment." in: Journal of virology, Vol. 76, Issue 3, pp. 1109-23, 2002 (PubMed).

Dzionek, Fuchs, Schmidt, Cremer, Zysk, Miltenyi, Buck, Schmitz: "BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 11, pp. 6037-46, 2000 (PubMed).

Target Details for Integrin beta 3

Target:

Integrin beta 3 (ITGB3)

Alternative Name: CD61 (ITGB3 Antibody Abstract)

Synonyms: BDPLT16, BDPLT2, CD61, GP3A, GPIIIa, GT, INGRB3, integrin, INTB3, GPIIIA, cd61, gp3a, itgb3, itgb3.2, integrin subunit beta 3, integrin beta 3, integrin subunit beta 3 L homeolog, integrin beta 3a, ITGB3, Itgb3, itgb3.L, itgb3a

Background: CD61 (beta3 integrin) is a transmembrane glycoprotein, which associates with CD41 or CD51 Molecules to form heterodimeric adhesion receptores. CD41/CD61 complex is one of the earliest markers of the megakaryocytic lineage. It binds to fibronectin, fibrinogen and von Willebrand factor, and is involved in platelet aggregation. CD51/CD61 complex has similar binding properties and is involved in modulating migration and survival of angiogenic endothelial cells.

Gene ID: 3690

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex