Lhcii Type II Chlorophyll A/B-Binding Protein (Lhcb2) antibody

Details for Product No. ABIN93509
Antigen
Reactivity
Arabidopsis thaliana, Barley (Hordeum vulgare), Chlamydomonas reinhardtii (C. reinhardtii), Green bean (Phaseolus vulgaris), Maize/Corn (Zea mays), Moss (Physcomitrella patens), Pea (Pisum sativum), Rice (Oryza sativa), Spinach (Spinacia oleracea), Tobacco (Nicotiana tabacum)
Host
Rabbit
Clonality
Polyclonal
Application
Immunoprecipitation (IP), Western Blotting (WB)
Options
Immunogen BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhcb2 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana Lhcb2.1 UniProt: Q9SHR7, TAIR: At2g05100, Lhcb2.2 UniProt: Q9S7J7, TAIR:At2g05070, Lhcb2.3 UniProt:Q9XF87, TAIR:At2g27690
Isotype IgG
Characteristics Expected / apparent Molecular Weight of the Antigene: 25 / 25 kDa for Arabidopsis thaliana
Purification affinity purified
Antigen
Background AGI Code: At2g05100
The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb2 is often coded by several nuclear genes and is found together with Lhcb1 within the mobile LHCII trimers involved in state1-state2 transition.A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII: Differential Analysis of the Lhcb1-3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467-9476.
Molecular Weight expected: 25 kDa, apparent: 25 kDa for Arabidopsis thaliana
UniProt Q9XF86, Q9XF87, Q9SHR7, Q9S7J7
Application Notes Recommended Dilution: 1 : 5000 with standard ECL (WB).
Comment

Immunoprecipitation has been done using Immunoprecipitation kit from Roche, Cat.No. 11 719 386 001.

Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 200 µL of sterile water
Buffer PBS pH 7.4
Handling Advice Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Storage -20 °C
Product cited in: Scotti, Sannino, Idoine, Hamman, De Stradis, Giorio, Maréchal-Drouard, Bock, Cardi: "The HIV-1 Pr55(gag) polyprotein binds to plastidial membranes and leads to severe impairment of chloroplast biogenesis and seedling lethality in transplastomic tobacco plants." in: Transgenic research, 2014 (PubMed).

Albus, Salinas, Czarnecki, Kahlau, Rothbart, Thiele, Lein, Bock, Grimm, Schöttler: "LCAA, a novel factor required for magnesium protoporphyrin monomethylester cyclase accumulation and feedback control of aminolevulinic acid biosynthesis in tobacco." in: Plant physiology, Vol. 160, Issue 4, pp. 1923-39, 2012 (PubMed).

Yuan, Xu, Yuan, Chen, Du, Xu, Zhang, Guo, Zhao, Lin: "Light regulation to chlorophyll synthesis and plastid development of the chlorophyll-less golden-leaf privet." in: Journal of integrative plant biology, Vol. 52, Issue 9, pp. 809-16, 2010 (PubMed).

Tikkanen, Nurmi, Kangasjaervi, Aro: "Core protein phosphorylation facilitates the repair of photodamaged photosystem II at high light." in: Biochimica et biophysica acta, 2008 (PubMed).

Tikkanen, Nurmi, Suorsa, Danielsson, Mamedov, Styring, Aro: "Phosphorylation-dependent regulation of excitation energy distribution between the two photosystems in higher plants." in: Biochimica et biophysica acta, Vol. 1777, Issue 5, pp. 425-32, 2008 (PubMed).