CD16 Molecule (CD16) antibody

Details for Product No. ABIN93988
Antigen
Reactivity
Human, Non-Human Primate
481
60
30
18
16
15
10
10
6
6
6
3
3
2
2
Host
Mouse
461
45
35
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
80
66
50
25
14
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
471
118
107
80
71
51
36
25
13
10
7
5
4
4
4
2
2
2
2
1
Options
Immunogen Normal human peripheral blood granulocytes
Clone LNK16
Isotype IgG1
Specificity The antibody LNK16 reacts with CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA, 50-65 kDa, expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB, 48 kDa, mainly expressed on neutrophils).
HLDA V, WS Code M MA069
HLDA V, WS Code NK NK50
Cross-Reactivity (Details) Human, Non-Human Primates
Purification Purified by precipitation and chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD16 (CD16 Antibody Abstract)
Background CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms –, FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD16 Molecule (CD16) antibody (ABIN93988) Surface staining of human peripheral blood cells with anti-CD16 (LNK16) PE.
Product cited in: Hovden, Karlsen, Jonsson, Aarstad, Appel: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." in: BMC immunology, Vol. 12, pp. 2, 2011 (PubMed).

Boyle: "Human macrophages kill human mesangial cells by Fas-L-induced apoptosis when triggered by antibody via CD16." in: Clinical and experimental immunology, Vol. 137, Issue 3, pp. 529-37, 2004 (PubMed).

Mathison, Befus, Davison, Woodman: "Modulation of neutrophil function by the tripeptide feG." in: BMC immunology, Vol. 4, pp. 3, 2003 (PubMed).

Tamm, Schmidt: "The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 4, pp. 1576-81, 1996 (PubMed).