CD16 Molecule (CD16) antibody (FITC)

Details for Product No. ABIN93995
Antigen
Reactivity
Human
481
60
30
18
16
15
11
10
6
6
6
3
3
2
2
Host
Mouse
461
45
35
1
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
79
66
50
25
14
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunoprecipitation (IP), Western Blotting (WB)
471
118
107
79
70
51
36
25
13
10
7
5
4
4
4
2
2
2
2
1
Options
Immunogen Human granulocytes
Clone MEM-154
Isotype IgG1
Specificity The antibody MEM-154 reacts with an epitope on CD16 antigen that is residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgammaRIII antigen). The antibody MEM-154 reacts with CD16+ granulocytes.
HLDA V, WS Code M MA068
HLDA V, WS Code NK NK51
Cross-Reactivity (Details) Human
Antigen
Alternative Name CD16 (CD16 Antibody Abstract)
Background CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms –, FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD16 Molecule (CD16) antibody (FITC) (ABIN93995) Surface staining of human peripheral blood cells with anti-human CD16 (MEM-154) FITC.
Product cited in: Reil, Sachs, Siahanidou, Flesch, Bux: "HNA-1d: a new human neutrophil antigen located on Fcγ receptor IIIb associated with neonatal immune neutropenia." in: Transfusion, 2013 (PubMed).

Gasdaska, Sherwood, Regan, Dickey: "An afucosylated anti-CD20 monoclonal antibody with greater antibody-dependent cellular cytotoxicity and B-cell depletion and lower complement-dependent cytotoxicity than rituximab." in: Molecular immunology, Vol. 50, Issue 3, pp. 134-41, 2012 (PubMed).

Schnueriger, Grau, Sondermann, Schreitmueller, Marti, Zocher: "Development of a quantitative, cell-line based assay to measure ADCC activity mediated by therapeutic antibodies." in: Molecular immunology, Vol. 48, Issue 12-13, pp. 1512-7, 2011 (PubMed).

Koene, Kleijer, Algra, Roos, von dem Borne, de Haas: "Fc gammaRIIIa-158V/F polymorphism influences the binding of IgG by natural killer cell Fc gammaRIIIa, independently of the Fc gammaRIIIa-48L/R/H phenotype." in: Blood, Vol. 90, Issue 3, pp. 1109-14, 1997 (PubMed).

de Haas, Koene, Kleijer, de Vries, Simsek, van Tol, Roos, von dem Borne: "A triallelic Fc gamma receptor type IIIA polymorphism influences the binding of human IgG by NK cell Fc gamma RIIIa." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 8, pp. 2948-55, 1996 (PubMed).

Tamm, Schmidt: "The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 4, pp. 1576-81, 1996 (PubMed).