CD63 antibody (CD63 Molecule) (PE)

Details for Product anti-CD63 Antibody No. ABIN94216
Antigen
Reactivity
Human
388
111
23
5
4
4
3
3
3
3
3
3
3
3
3
3
2
1
Host
Mouse
306
107
4
4
Clonality (Clone)
Monoclonal ()
Conjugate
This CD63 antibody is conjugated to PE
32
30
25
19
7
6
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
266
176
120
116
78
70
63
51
48
26
22
12
7
7
4
2
1
Options
Immunogen HPB-ALL T cell line
Clone MEM-259
Isotype IgG1
Specificity The antibody MEM-259 reacts with CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.
Cross-Reactivity (Details) Human
Antigen
Alternative Name CD63 (CD63 Antibody Abstract)
Background CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD63 Molecule (CD63) antibody (PE) (ABIN94216) Immunofluorescence staining of CD63 in human HeLa cell line using anti-CD63
Image no. 2 for anti-CD63 Molecule (CD63) antibody (PE) (ABIN94216) Flow cytometry analysis of peripheral blood lymphocytes from a patient with allergy t...
Image no. 3 for anti-CD63 Molecule (CD63) antibody (PE) (ABIN94216) Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green...
Image no. 4 for anti-CD63 Molecule (CD63) antibody (PE) (ABIN94216) Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green...
Product cited in: Heneberg, Riegerová, Kučera: "Pimecrolimus Is a Potent Inhibitor of Allergic Reactions to Hymenopteran Venom Extracts and Birch Pollen Allergen In Vitro." in: PLoS ONE, Vol. 10, Issue 11, pp. e0142953, 2015 (PubMed).

Cerny, Feng, Yu, Miyake, Borgonovo, Klumperman, Meldolesi, McNeil, Kirchhausen: "The small chemical vacuolin-1 inhibits Ca(2+)-dependent lysosomal exocytosis but not cell resealing." in: EMBO reports, Vol. 5, Issue 9, pp. 883-8, 2004 (PubMed).