Clusterin antibody
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- Target See all Clusterin (CLU) Antibodies
- Clusterin (CLU)
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Clusterin antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- The mouse monoclonal antibody Hs-3 recognizes human clusterin (apolipoprotein J), a conserved secreted glycoprotein.
- No Cross-Reactivity
- Cat, Cow, Dog, Pig
- Cross-Reactivity (Details)
- Human
- Purification
- Purified by protein-A affinity chromatography.
- Purity
- > 95 % (by SDS-PAGE)
- Immunogen
- Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
- Clone
- Hs-3
- Isotype
- IgG1
- Top Product
- Discover our top product CLU Primary Antibody
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- Application Notes
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Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: Sertoli cells.
Western blotting: Recommended dilution: 1 μg/mL, positive material: seminal plasma. - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze.
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New monoclonal antibody to human apolipoprotein J." in: Folia biologica, Vol. 48, Issue 1, pp. 40-2, (2002) (PubMed).
: "
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New monoclonal antibody to human apolipoprotein J." in: Folia biologica, Vol. 48, Issue 1, pp. 40-2, (2002) (PubMed).
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- Target
- Clusterin (CLU)
- Alternative Name
- Clusterin (CLU Products)
- Background
- Clusterin,Clusterin (APO J, SGP-2, TRPM-2, SP-40, pADHC-9, CLJ, T64, GP III, XIP8) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30 % of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified. It is a conserved secreted glycoprotein expressed by a wide range of tissues and being implicated in many physiological processes, including e.g. lipid transportation, complement inhibition, tissue remodeling, membrane recycling, or clearence of cellular debris. It is nearly ubiqitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. Clusterin is able to bind and form complexes with numerous partners (immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin etc.) and is expressed in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others. A genuine function of clusterin is still enigmatic.,Complement-associated protein SP-40, Complement cytolysis inhibitor, CLI, CLU, NA1/NA2, Apolipoprotein J, Apo-J, TRPM-2, KBP1, KUB1, CLI, AAG4, APOJ, SGP2
- Gene ID
- 1191
- UniProt
- P10909
- Pathways
- Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
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