Clusterin antibody (CLU)

Details for Product anti-CLU Antibody No. ABIN94264
Antigen
Reactivity
Human
242
60
52
18
11
7
3
2
2
1
1
Host
Mouse
198
82
30
10
4
2
Clonality (Clone)
Monoclonal ()
Conjugate
This Clusterin antibody is un-conjugated
40
31
9
8
7
6
3
3
3
3
3
3
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
272
177
133
45
35
34
33
20
15
14
9
7
2
1
Options
Immunogen Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Clone Hs-3
Isotype IgG1
No Cross-Reactivity Pig (Porcine), Cow (Bovine), Dog (Canine), Cat (Feline)
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name Clusterin (CLU Antibody Abstract)
Background Clusterin (APO J, SGP-2, TRPM-2, SP-40, pADHC-9, CLJ, T64, GP III, XIP8) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30 % of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified. It is a conserved secreted glycoprotein expressed by a wide range of tissues and being implicated in many physiological processes, including e.g. lipid transportation, complement inhibition, tissue remodeling, membrane recycling, or clearence of cellular debris. It is nearly ubiqitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. Clusterin is able to bind and form complexes with numerous partners (immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin etc.) and is expressed in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others. A genuine function of clusterin is still enigmatic.
Pathways Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Product cited in: Capková, Geussová, Peknicová: "New monoclonal antibody to human apolipoprotein J." in: Folia biologica, Vol. 48, Issue 1, pp. 40-2, 2002 (PubMed).