TCR beta (Vbeta5.3-Related) antibody

Details for Product No. ABIN94477
Antigen
Reactivity
Human
2
Host
Mouse
2
Clonality (Clone)
Monoclonal ()
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
2
2
2
2
Options
Immunogen Human thymoma cell line HPB-ALL.
Clone MEM-262
Isotype IgG2a
Specificity The antibody MEM-262 recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.
Cross-Reactivity (Details) Other not tested, Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-TCR beta (Vbeta5.3-Related) antibody (ABIN94477) Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-...
Product cited in: Pavlistová, Drbal, Hilgert, Horejsí: "A novel monoclonal reagent recognizing native and denatured Vbeta5.3-related chains of human T cell receptor." in: Immunology letters, Vol. 88, Issue 2, pp. 105-8, 2003 (PubMed).