Myeloperoxidase antibody

Catalog No. ABIN964728

Supplier Product No.: 200-4181

Product Details anti-Myeloperoxidase Antibody

Target: Myeloperoxidase (MPO)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Myeloperoxidase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 200-4181

Supplier: Rockland

Purpose: Myeloperoxidase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Myeloperoxidase [Human Leukocytes].

Characteristics: Synonyms: rabbit anti-Myeloperoxidase Antibody, rabbit anti-MPO antibody

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Myeloperoxidase [Human Leukocytes]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This antibody is tested by western blotting suitable for ELISA and immunoprecipitation.  Although not tested, this antibody is likely functional in immunohistochemistry and other immunological methods.  Anti-Human Myeloperoxidase may react with MPO from other sources. Anti-Human Myeloperoxidase detects neutrophilic granulocytes and monocytes in blood and precursors of granulocytes in the bone marrow. This antibody may also detect myeloid leukemias of the bone marrow as well as other sites.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 5.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Myeloperoxidase

Target: Myeloperoxidase (MPO)

Alternative Name: MPO (MPO Products)

Background: Background: Human myeloperoxidase (MPO) is a dimeric protein composed of two heavy subunits (53 kDa) and two light subunits (15 kDa).  Each MPO molecule contains two prosthetic porphyrins which play an important role in the catalytic cycle.  Molecular weights for MPO isoforms from pools of normal human samples range from 114,000 to 140,000 daltons reflecting a heterogeneous mixture of isoforms when assayed under non-reducing conditions of SDS-PAGE.  Often MPO from a single donor will yield a homogenous preparation reflecting a single isoform.  The carbohydrate component of MPO, consisting of mannose, glucose and N-acetylglucosamine residues is 2.5 % . MPO is inhibited by azide and other compounds.  MPO is stored in primary granules of neutrophils and serves as a bactericidal agent in that MPO catalyzes the production of hypochlorous acid (HOCl), a powerful oxidant.  HOCl is derived from chloride ion (Cl-) and hydrogen peroxide (H₂O₂).  In a number of inflammatory situations, MPO is released into the extracellular matrix where its measurement can be used as an indication of neutrophil activation.

Gene ID: 4353

UniProt: P05164

Pathways: Chromatin Binding