RBC Lysis Buffer (10X)

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Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)
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Characteristics Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer is supplied as a 10X solution containing ammonium chloride, potassium carbonate, and EDTA, and should be diluted in deionized water prior to use. Nucleated RBCs are not effectively lysed with ammonium chloride.
Components RBC Lysis Buffer (10X)
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 10 X
Buffer This red blood cell (RBC) lysis buffer is supplied as a 10X solution and should be diluted to 1X in deionized water. 100 mLof 10X concentrate will yield a quantity of 1X solution that is sufficient to lyse 500 samples. The pH of the 1X solution should fall within the range of pH 7.1-7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use.
Storage 4 °C
Storage Comment Store RBC lysis buffer between 2°C and 8°C.
Background publications Chang, Tien, Wen, Hsieh, Hwang: "Recovery of CD45(-)/Lin(-)/SSEA-4(+) very small embryonic-like stem cells by cord blood bank standard operating procedures." in: Cytotherapy, Vol. 16, Issue 4, pp. 560-5, 2014 (PubMed).

Kim, Nagy, Park et al.: "Globular adiponectin inhibits ethanol-induced reactive oxygen species production through modulation of NADPH oxidase in macrophages: involvement of liver kinase B1/AMP-activated protein kinase ..." in: Molecular pharmacology, Vol. 86, Issue 3, pp. 284-96, 2014 (PubMed).

Cekic, Day, Sag, Linden: "Myeloid expression of adenosine A2A receptor suppresses T and NK cell responses in the solid tumor microenvironment." in: Cancer research, Vol. 74, Issue 24, pp. 7250-9, 2014 (PubMed).

Kraft, Göb, Schuhmann, Göbel, Deppermann, Thielmann, Herrmann, Lorenz, Brede, Stoll, Meuth, Nieswandt, Pfeilschifter, Kleinschnitz: "FTY720 ameliorates acute ischemic stroke in mice by reducing thrombo-inflammation but not by direct neuroprotection." in: Stroke; a journal of cerebral circulation, Vol. 44, Issue 11, pp. 3202-10, 2013 (PubMed).

Fullerton, Roth, Ganey: "Pretreatment with TCDD exacerbates liver injury from Concanavalin A: critical role for NK cells." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 136, Issue 1, pp. 72-85, 2013 (PubMed).

Saraiva, Alves e Silva, Correia Silva, da Costa, Gollob, Dutra, Moreira: "Evaluation of IL17A expression and of IL17A, IL17F and IL23R gene polymorphisms in Brazilian individuals with periodontitis." in: Human immunology, Vol. 74, Issue 2, pp. 207-14, 2013 (PubMed).

Cekic, Sag, Day, Linden: "Extracellular adenosine regulates naive T cell development and peripheral maintenance." in: The Journal of experimental medicine, Vol. 210, Issue 12, pp. 2693-706, 2013 (PubMed).

Ma, Suthar, Kasahara, Gale, Clark: "CD22 is required for protection against West Nile virus Infection." in: Journal of virology, Vol. 87, Issue 6, pp. 3361-75, 2013 (PubMed).

Varga, Mounier, Gogolak, Poliska, Chazaud, Nagy: "Tissue LyC6- macrophages are generated in the absence of circulating LyC6- monocytes and Nur77 in a model of muscle regeneration." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 11, pp. 5695-701, 2013 (PubMed).

Han, Li, Cai, Gleaves, Polosukhin, Segal, Yull, Blackwell: "NADPH oxidase limits lipopolysaccharide-induced lung inflammation and injury in mice through reduction-oxidation regulation of NF-κB activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 190, Issue 9, pp. 4786-94, 2013 (PubMed).

Garcia-Arcos, Hiyama, Drosatos, Bharadwaj, Hu, Son, OByrne, Chang, Deckelbaum, Takahashi, Westerterp, Obunike, Jiang, Yagyu, Blaner, Goldberg: "Adipose-specific lipoprotein lipase deficiency more profoundly affects brown than white fat biology." in: The Journal of biological chemistry, Vol. 288, Issue 20, pp. 14046-58, 2013 (PubMed).

Yamasaki, Miura, Davydova, Vickers, Yamamoto: "A single intraduodenal administration of human adenovirus 40 vaccine effectively prevents anaphylactic shock." in: Clinical and vaccine immunology : CVI, Vol. 20, Issue 10, pp. 1508-16, 2013 (PubMed).

Deiuliis, Kampfrath, Zhong, Oghumu, Maiseyeu, Chen, Sun, Satoskar, Rajagopalan: "Pulmonary T cell activation in response to chronic particulate air pollution." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 302, Issue 4, pp. L399-409, 2012 (PubMed).

Hsia, Ledford, Potts-Kant, Nikam, Lugogo, Foster, Kraft, Abraham, Wright: "Mast cell TNF receptors regulate responses to Mycoplasma pneumoniae in surfactant protein A (SP-A)-/- mice." in: The Journal of allergy and clinical immunology, Vol. 130, Issue 1, pp. 205-14.e2, 2012 (PubMed).

Rico, Laguë, Lefèvre, Tsoi, Dodelet-Devillers, Kumar, Lapointe, Paquet, Nadeau, Boerboom: "Pharmacological targeting of mammalian target of rapamycin inhibits ovarian granulosa cell tumor growth." in: Carcinogenesis, Vol. 33, Issue 11, pp. 2283-92, 2012 (PubMed).

Jin, Arif, Garcia-Fernandez, Ennis, Davis, Thompson, Curci: "Novel mechanism of aortic aneurysm development in mice associated with smoking and leukocytes." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 32, Issue 12, pp. 2901-9, 2012 (PubMed).

Roybal, Zang, Ahn, Yang, Gibbons, Baird, Alvarez, Thilaganathan, Liu, Saintigny, Heymach, Creighton, Kurie: "miR-200 Inhibits lung adenocarcinoma cell invasion and metastasis by targeting Flt1/VEGFR1." in: Molecular cancer research : MCR, Vol. 9, Issue 1, pp. 25-35, 2011 (PubMed).

Kahlenberg, Thacker, Berthier, Cohen, Kretzler, Kaplan: "Inflammasome activation of IL-18 results in endothelial progenitor cell dysfunction in systemic lupus erythematosus." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 11, pp. 6143-56, 2011 (PubMed).

de Resende, Stodola, Greene: "Role of the renin angiotensin system on bone marrow-derived stem cell function and its impact on skeletal muscle angiogenesis." in: Physiological genomics, Vol. 42, Issue 3, pp. 437-44, 2010 (PubMed).

Adams, Bauer, Flick, Sikorski, Nuriel, Lassmann, Degen, Akassoglou: "The fibrin-derived gamma377-395 peptide inhibits microglia activation and suppresses relapsing paralysis in central nervous system autoimmune disease." in: The Journal of experimental medicine, Vol. 204, Issue 3, pp. 571-82, 2007 (PubMed).