SensoLyte® 520 Cathepsin G Assay Kit

Details for Product No. ABIN1882442
Antigen
Detection Method
Fluorometric
Application
Fluorescence Resonance Energy Transfer Microscopy (FRET)
Options
Brand SensoLyte®
Detection Method Fluorometric
Characteristics The SensoLyte® 520 Cathepsin G Activity Assay Kit uses a HiLyte Fluor™ 488/QXL™ 520 FRET peptide substrate for the measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte Fluor™ 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490/520 nm. With superior fluorescence quantum yield and longer emission wavelength, the HiLyte Fluor™ 488/QXL™ 520 based FRET peptide has less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The fluorescent signal from HiLyte Fluor™ 488 is stable at low pH, which is the optimal pH for cathepsin activity. The kit can be used to detect the activity of Cathepsin G enzyme in biological samples and purified enzyme preparations.
Target Name (Antigen)
Alternative Name Cathepsin G (CTSG ELISA Kit Abstract)
Background Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin G is the serine protease released by neutrophils upon their activation. Cathepsin G is currently being explored as a target for anti-inflammatory and anti-infective drugs.
Comment

FRET-based Assay Kit

Restrictions For Research Use only
Handling Advice Protect Components A and B from light and moisture.
Storage -80 °C
Storage Comment Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience
Supplier Images
Image no. 1 for SensoLyte® 520 Cathepsin G Assay Kit (ABIN1882442) SensoLyte® 520 Cathepsin G Assay Kit
Product cited in: Wågsäter, Johansson, Fontaine, Vorkapic, Bäcklund, Razuvaev, Mäyränpää, Hjerpe, Caidahl, Hamsten, Franco-Cereceda, Wilbertz, Swedenborg, Zhou, Eriksson: "Serine protease inhibitor A3 in atherosclerosis and aneurysm disease." in: International journal of molecular medicine, Vol. 30, Issue 2, pp. 288-94, 2012 (PubMed).