Adrenaline (Epinephrine) ELISA Kit

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Sample Type Urine, Plasma
Detection Method Colorimetric
Sensitivity Urine: 0.16 ng/ml, Plasma: 8 pg/ml
Characteristics Catecholamine is the name of a group of aromatic amines (noradrenaline, adrenaline, dopamine, and their derivatives) which act as hormones and neurotransmitters, respectively. Adrenaline and noradrenaline are formed from dopamine. They act on the cardiac musculature and the metabolism (adrenaline) as well as on the peripheral circulation (noradrenaline) and help the body to cope with acute and chronic stress.
Application Notes Optimal working dilution should be determined by the investigator.
Sample Volume 15 μL
Plate Pre-coated
Protocol The competitive Adrenaline (Epinephrine) ELISA Kit uses the microtiter plate format. Adrenaline is bound to the solid phase of the microtiter plate. Acylated adrenaline from the sample and solid phase bound adrenaline compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase adrenaline is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase adrenaline is inversely proportional to the adrenaline concentration of the sample.
Restrictions For Research Use only
Storage 4 °C
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