×
For best experience we recommend to activate Javascript in your browser.
Tissue factor ELISA Kit
F3
Reactivity: Mouse
Colorimetric
Sandwich ELISA
1.6-400 ng/mL
Cell Culture Supernatant, Plasma, Serum
Product Details Tissue factor ELISA Kit
(hide)
Target
See all Tissue factor (F3) ELISA Kits
Tissue factor (F3)
(Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
Reactivity
All reactivities for Tissue factor elisa kits
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
1.6-400 ng/mL
Minimum Detection Limit
1.6 ng/mL
Application
ELISA
Purpose
Mouse Coagulation Factor III (Tissue Factor/Thromboplastin) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Sample Type
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
Specificity
The antibody pair provided in this kit recognizes Mouse Coagulation Factor III (F3 / Tissue Factor / Thromboplastin)
Sensitivity
1.6 ng/mL
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Featured
Discover our best selling F3 ELISA Kit
Top Product
Discover our top product F3 ELISA Kit
Alternatives
(show)
Application Details
(hide)
Application Notes
Recommended Dilution for serum and plasma samples2 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
(hide)
Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for Tissue factor
(hide)
Target
See all Tissue factor (F3) ELISA Kits
Tissue factor (F3)
(Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
Alternative Name
Coagulation Factor III (F3 Products )
Synonyms
CD142 ELISA Kit, TF ELISA Kit, TFA ELISA Kit, PRO1557 ELISA Kit, PRO2086 ELISA Kit, TFQTL1 ELISA Kit, f3 ELISA Kit, AA409063 ELISA Kit, Cf-3 ELISA Kit, Cf3 ELISA Kit, tf ELISA Kit, zgc:112151 ELISA Kit, coagulation factor III, tissue factor ELISA Kit, transferrin ELISA Kit, coagulation factor IIIa ELISA Kit, coagulation factor III ELISA Kit, tissue factor ELISA Kit, coagulation factor IIIb ELISA Kit, coagulation factor III (thromboplastin, tissue factor) S homeolog ELISA Kit, F3 ELISA Kit, TF ELISA Kit, f3a ELISA Kit, tf ELISA Kit, f3b ELISA Kit, f3.S ELISA Kit
Background
Gene Names: F3 Cf-3 Cf3 Protein names: Tissue factor (TF) (Coagulation factor III) (CD antigen CD142)
Gene ID
14066
UniProt
P20352
Pathways
Positive Regulation of Endopeptidase Activity , Smooth Muscle Cell Migration , Platelet-derived growth Factor Receptor Signaling
Recently viewed
(hide)