EIF2B1 ELISA Kit
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- Target See all EIF2B1 ELISA Kits
- EIF2B1 (Eukaryotic Translation Initiation Factor 2B, Subunit 1 Alpha, 26kDa (EIF2B1))
- Binding Specificity
- pSer52
- Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- Human Phospho-EIF2A (S52) ELISA Kit. This assay semi-quantitatively measures phophorylated EIF2A (Ser52) in lysate samples.
- Sample Type
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- Specificity
- The antibody pair provided in this kit recognizes human eIF-2A phosphorylated at site Serine-52.
- Characteristics
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- Material not included
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Assay Procedure
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Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately. - Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- Expiry Date
- 6 months
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- Target See all EIF2B1 ELISA Kits
- EIF2B1 (Eukaryotic Translation Initiation Factor 2B, Subunit 1 Alpha, 26kDa (EIF2B1))
- Alternative Name
- eIF-2a (EIF2B1 Products)
- Synonyms
- DDBDRAFT_0219393 ELISA Kit, DDBDRAFT_0231375 ELISA Kit, DDB_0219393 ELISA Kit, DDB_0231375 ELISA Kit, 26kDa ELISA Kit, D5Ertd406e ELISA Kit, EIF2B ELISA Kit, EIF2BA ELISA Kit, eIF-2a ELISA Kit, translation initiation factor eIF-2B alpha subunit ELISA Kit, hypothetical protein ELISA Kit, Translation initiation factor eIF-2B subunit alpha ELISA Kit, eukaryotic translation initiation factor 2B subunit alpha ELISA Kit, eukaryotic translation initiation factor 2B, subunit 1 (alpha) ELISA Kit, eIF2b1 ELISA Kit, PGTG_05021 ELISA Kit, ei2ba ELISA Kit, eif2b1 ELISA Kit, Eif2b1 ELISA Kit, EIF2B1 ELISA Kit
- Background
- Eukaryotic translation initiation factor 2A (eIF-2A) phosphorylated at Serine-52
- Gene ID
- 1965
- UniProt
- P05198
- Pathways
- Methionine Biosynthetic Process
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