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Interleukin 37 (IL37) ELISA Kit ELISA Kit

IL37 Reactivity: Human AA 46-218 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant
Pubmed (2)
Catalog No. ABIN2859232
$369.00
Plus shipping costs $45.00
96 tests
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  • Target
    Binding Specificity
    AA 46-218
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    15.6-1000 pg/mL
    Minimum Detection Limit
    15.6 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-37
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: E.coli
    Immunogen sequence: V46-D218
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: V46-D218
  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Belongs to the IL-1 family.

    Tissue Specificity: In general, low constitutive expression, if any, in healthy tissues, high expression in inflammatory counterparts, including in synovial tissues from individuals with active rheumatoid arthritis. Isoform A, isoform B and isoform C are expressed in testis, colon, placenta, lung and lymph node. Isoform D and isoform E were found only in testis and bone marrow. Whereas only isoform A is found in brain, only isoform B in kidney and only isoform C in heart. .

    Plate
    Pre-coated
    Protocol
    human IL-37 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-37 has been precoated onto 96-well plates. Standards(E.coli, V46-D218) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-37 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-37 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL human IL-37 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human IL-37 standard solution and each sample is measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 37.7, Standard deviation: 1.39, CV(%): 3.7
    • Sample 2: n=16, Mean(pg/ml): 118, Standard deviation: 5.43, CV(%): 4.6
    • Sample 3: n=16, Mean(pg/ml): 621, Standard deviation: 32.91, CV(%): 5.3,
    • Sample 1: n=24, Mean(pg/ml): 216, Standard deviation: 12.3, CV(%): 5.7
    • Sample 2: n=24, Mean(pg/ml): 365, Standard deviation: 15.33, CV(%): 4.2
    • Sample 3: n=24, Mean(pg/ml): 610, Standard deviation: 39.04, CV(%): 6.4
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • DAngelo, Reale, Costantini, Di Nicola, Porfilio, de Andrés, Fernández-Paredes, Sánchez-Ramón, Pasquali: "Profiling of Canonical and Non-Traditional Cytokine Levels in Interferon-β-Treated Relapsing-Remitting-Multiple Sclerosis Patients." in: Frontiers in immunology, Vol. 9, pp. 1240, 2018 (PubMed).

    Yu, Lu, Li, Xiao, Xing, Li, Wu: "Role of S100A1 in hypoxia-induced inflammatory response in cardiomyocytes via TLR4/ROS/NF-?B pathway." in: The Journal of pharmacy and pharmacology, Vol. 67, Issue 9, pp. 1240-50, 2015 (PubMed).

  • Target
    Alternative Name
    IL37 (IL37 ELISA Kit Abstract)
    Synonyms
    FIL1, FIL1(ZETA), FIL1Z, IL-1F7, IL-1H, IL-1H4, IL-1RP1, IL-37, IL1F7, IL1H4, IL1RP1, interleukin 37, IL37
    Background

    Protein Function: Suppressor of innate inflammatory and immune responses involved in curbing excessive inflammation. This function requires SMAD3. Suppresses, or reduces, proinflammatory cytokine production, including IL1A and IL6, as well as CCL12, CSF1, CSF2, CXCL13, IL1B, IL23A and IL1RN, but spares anti-inflammatory cytokines. Inhibits dendritic cell activation. .

    Background: Interleukin 37(IL-37) also named IL1F7, is a protein that in humans is encoded by the IL1F7 gene. The protein encoded by this gene is a member of the interleukin 1 cytokine family. And it is mapped to 2q13. In vitro expression analysis showed that IL-37 was a secretory protein. And IL-37 could play an important role in the link between innate and adaptive immunity and may be useful for tumor immunotherapy. Meanwhile, IL-37 inhibits the production of inflammatory cytokines in peripheral blood mononuclear cells of patients with systemic lupus erythematosus. In addition, IL-37 can emerged as a dual-function cytokine with intra- and extracellular properties for suppressing innate inflammation. Furthermore, IL-37 is a new anti-inflammatory cytokine that plays an important role in protecting against tissue injury during infections via limiting immune and inflammatory reactions.

    Synonyms: Interleukin-37,FIL1 zeta,IL-1X,Interleukin-1 family member 7,IL-1F7,Interleukin-1 homolog 4,IL-1H,IL-1H4,Interleukin-1 zeta,IL-1 zeta,Interleukin-1-related protein,IL-1RP1,Interleukin-23,IL-37,IL37,FIL1Z, IL1F7, IL1H4, IL1RP1,

    Full Gene Name: Interleukin-37

    Cellular Localisation: Cytoplasm, cytosol. Nucleus. Secreted. Stimulation with IL1B leads to colocalization with SMAD3 mostly in perinuclear regions. Only the CASP1-cleaved mature form translocates into the nucleus upon LPS stimulation.
    Gene ID
    27178
    UniProt
    Q9NZH6
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