- Enzyme Solution:
a. Dissolve lyophilized Enzyme Reagent in 10 mL of purified water.
b. Filter solution with a 0.45 µm membrane filter.
c. Aliquot and store the enzyme solution at -20°C until needed. The frozen enzyme solution is stable for 3 months. Avoid repeat freeze/thaw cycles.
2. Calibrator Solution:
a. Serial dilute the Chondroitin Sulfate Calibrator (100 µg/mL) in sterile purified water to 100, 50, 25, 12.5, 6.3, and 3.1 µg/mL.
b. The diluted calibrator can be stored frozen at -20°C for 1 year.
3. Staining Solution:
a. Prepare Staining Stock Solution just prior to performing the assay. Color of the diluted staining stock solution may fade in 5 seconds - 5 minutes. Fading is normal and does not affect the performance of the solution.
b. Mix 0.4 mL of Staining Stock Solution to 12.6 mL of Buffer. Protect from light.