Acidic Mucopolysaccharide Assay Kit

Details for Product No. ABIN3170545
Antigen
Detection Method
Colorimetric
Application
Immunoassay (IA)
Options
Detection Method Colorimetric
Components Staining Stock Solution: 1 bottle, 4 mL
Buffer: 1 bottle, 130 mL
Enzyme Reagent: 5 vials, 10 mL/vial (lyophilized)
Chondroitin Sulfate Calibrator (100 µg/mL): 1 vial, 2 mL
There are enough reagents to run 100 assays.
Material not included Purified water
Spectrophotometer with 650 nm wavelength
Tubes
Centrifuge
Heater set at 60°C
0.45 µm membrane filter
Adjustable pipettor
Target Name (Antigen)
Background Mucopolysaccharides are a class of polysaccharides found in connective tissue that provides structural integrity to animals. The Acidic Mucopolysaccharide Kit is a simple and fast assay for the quantitative colorimetric determination of acidic mucopolysaccharides in animal cartilage tissue or cultured chondrocytes (cell layers or spheroid cell clusters). Cartilage tissue or cell layers are enzymatically treated to digest proteins and leave behind the acidic mucopolysaccharides. The acidic mucopolysaccharides are stained and the resulting blue color is measured by a spectrophotometer at wavelength 650 nm. Conventional methodologies for the quantification of acidic mucopolysaccharides incorporate radioisotopes. Our colorimetric assay provides a convenient alternative to using radioisotopes. The Acidic Mucopolysaccharide Kit is for research use only. Not for use in diagnostic procedures. The stain contained in the kit can differentiate between acid mucopolysaccharides and other acidic biological substances.
Reagent Preparation
  1. Enzyme Solution:
    a. Dissolve lyophilized Enzyme Reagent in 10 mL of purified water.
    b. Filter solution with a 0.45 µm membrane filter.
    c. Aliquot and store the enzyme solution at -20°C until needed. The frozen enzyme solution is stable for 3 months. Avoid repeat freeze/thaw cycles.
    2. Calibrator Solution:
    a. Serial dilute the Chondroitin Sulfate Calibrator (100 µg/mL) in sterile purified water to 100, 50, 25, 12.5, 6.3, and 3.1 µg/mL.
    b. The diluted calibrator can be stored frozen at -20°C for 1 year.
    3. Staining Solution:
    a. Prepare Staining Stock Solution just prior to performing the assay. Color of the diluted staining stock solution may fade in 5 seconds - 5 minutes. Fading is normal and does not affect the performance of the solution.
    b. Mix 0.4 mL of Staining Stock Solution to 12.6 mL of Buffer. Protect from light.
Restrictions For Research Use only
Handling Advice 1. Read the instructions carefully before beginning the assay.
2. This kit is for research use only, not for human or diagnostic use.
3. Great care has been taken to ensure the quality and reliability of this product. However, it is possible that in certain cases, unusual results may be obtained due to high levels of interfering factors.
Storage 4 °C
Storage Comment Kit components can be stored at 4°C until expiration date.
Expiry Date The expiry date is stated on the label.