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ANGPTL3 ELISA Kit

ANGPTL3 Reactivity: Human Colorimetric Sandwich ELISA 8.2-2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN4881825
  • Target See all ANGPTL3 ELISA Kits
    ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))
    Reactivity
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    8.2-2000 pg/mL
    Minimum Detection Limit
    8.2 pg/mL
    Application
    ELISA
    Purpose
    Human ANGPTL3 ELISA Kit for serum, plasma, and cell culture supernatant samples.
    Sample Type
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA kit shows no cross-reactivity with the following cytokines tested: human beta IG-H3, CA9, Cathepsin B, CD23, CD26 (DPPIV), CHI3L1, Coagulation Factor III, CTLA-4, Dkk-4, EDA-A2, Erythropoietin R, FGF-6, FGF-9, FOLH1 (PSMA), Gas 1, IGFBP-5, IL-38 (IL-1 F10), IL-36 Ra (IL-1 F5), IL-36 alpha (IL-1 F6), IL-37 (IL-1 F7), IL-36 beta (IL-1 F8), IL-36 gamma (IL-1 F9), IL-1 R5 (IL-18 R alpha), IL-10 R alpha, IL-17C, IL-18, IL-20, IL-34, IL-5 R alpha, Layilin, Leptin R, Marapsin, Mer, MMP-7, P-Cadherin, Prostasin, SIGIRR, TGF beta R3,TWEAK.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Application Notes
    Recommended Dilution for serum and plasma samples200 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Bassyouni, Sharaf, Wali, Mansour: "Clinical significance of Angiopoietin-1 in Behcet's disease patients with vascular involvement." in: Heart and vessels, Vol. 31, Issue 6, pp. 918-24, (2017) (PubMed).

  • Target See all ANGPTL3 ELISA Kits
    ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))
    Alternative Name
    ANGPTL3 (ANGPTL3 Products)
    Synonyms
    fb60e11 ELISA Kit, fb66h02 ELISA Kit, wu:fb60e11 ELISA Kit, wu:fb66h02 ELISA Kit, zgc:111943 ELISA Kit, ANGPTL3 ELISA Kit, LOC100230785 ELISA Kit, ANG-5 ELISA Kit, ANGPT5 ELISA Kit, ANL3 ELISA Kit, FHBL2 ELISA Kit, hypl ELISA Kit, angiopoietin-like 3 ELISA Kit, angiopoietin like 3 ELISA Kit, angptl3 ELISA Kit, ANGPTL3 ELISA Kit, Angptl3 ELISA Kit
    Gene ID
    27329
    UniProt
    Q9Y5C1
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