ANGPTL3 ELISA Kit (Angiopoietin-Like 3)

Details for Product ANGPTL3 ELISA Kit No. ABIN4881825
Antigen
  • fb60e11
  • fb66h02
  • wu:fb60e11
  • wu:fb66h02
  • zgc:111943
  • ANGPTL3
  • LOC100230785
  • ANG-5
  • ANGPT5
  • ANL3
  • FHBL2
  • hypl
  • angiopoietin-like 3
  • angiopoietin like 3
  • angptl3
  • ANGPTL3
  • Angptl3
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
7
5
4
3
2
1
1
1
1
1
1
1
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
8.2-2000 pg/mL
Minimum Detection Limit
8.2 pg/mL
Application
ELISA
Options
Purpose Human ANGPTL3 ELISA Kit for serum, plasma, and cell culture supernatant samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human beta IG-H3, CA9, Cathepsin B, CD23, CD26 (DPPIV), CHI3L1, Coagulation Factor III, CTLA-4, Dkk-4, EDA-A2, Erythropoietin R, FGF-6, FGF-9, FOLH1 (PSMA), Gas 1, IGFBP-5, IL-38 (IL-1 F10), IL-36 Ra (IL-1 F5), IL-36 alpha (IL-1 F6), IL-37 (IL-1 F7), IL-36 beta (IL-1 F8), IL-36 gamma (IL-1 F9), IL-1 R5 (IL-18 R alpha), IL-10 R alpha, IL-17C, IL-18, IL-20, IL-34, IL-5 R alpha, Layilin, Leptin R, Marapsin, Mer, MMP-7, P-Cadherin, Prostasin, SIGIRR, TGF beta R3,TWEAK.
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Plasmids, Primers & others Plasmids, Primers & others ANGPTL3 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name ANGPTL3 (ANGPTL3 ELISA Kit Abstract)
Gene ID 27329
UniProt Q9Y5C1
Application Notes Recommended Dilution for serum and plasma samples200 fold
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months
Product cited in: Bassyouni, Sharaf, Wali, Mansour: "Clinical significance of Angiopoietin-1 in Behcet's disease patients with vascular involvement." in: Heart and vessels, Vol. 31, Issue 6, pp. 918-24, 2017 (PubMed). (Sample species: Human). Further details: Sample: Plasma (Behcet's disease patients with vascular involvement)

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