Complement Factor H ELISA Kit

Catalog No. ABIN4882314

Product Details Complement Factor H ELISA Kit

Target: Complement Factor H (CFH)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Complement Factor H ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human Complement Factor H.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Complement Factor H

Target: Complement Factor H (CFH)

Alternative Name: Complement Factor H (CFH Products)

Synonyms: AHUS1 ELISA Kit, AMBP1 ELISA Kit, ARMD4 ELISA Kit, ARMS1 ELISA Kit, CFHL3 ELISA Kit, FH ELISA Kit, FHL1 ELISA Kit, HF ELISA Kit, HF1 ELISA Kit, HF2 ELISA Kit, HUS ELISA Kit, Mud-1 ELISA Kit, NOM ELISA Kit, Sas-1 ELISA Kit, Sas1 ELISA Kit, Fh ELISA Kit, CFH ELISA Kit, im:7147412 ELISA Kit, si:ch211-207o17.2 ELISA Kit, fH ELISA Kit, armd4 ELISA Kit, arms1 ELISA Kit, cfh ELISA Kit, cfhl3 ELISA Kit, fhl1 ELISA Kit, hf1 ELISA Kit, hf2 ELISA Kit, hus ELISA Kit, complement factor H ELISA Kit, complement component factor h ELISA Kit, complement factor H-related protein 5 ELISA Kit, complement factor H L homeolog ELISA Kit, CFH ELISA Kit, Cfh ELISA Kit, cfh ELISA Kit, LOC100399888 ELISA Kit, LOC100733902 ELISA Kit, cfh.L ELISA Kit

Gene ID: 3075

UniProt: P08603

Pathways: Complement System, Cellular Response to Molecule of Bacterial Origin