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NME4 ELISA Kit (NME/NM23 Nucleoside Diphosphate Kinase 4) ELISA Kit

NME4 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Cells
Catalog No. ABIN5564631
$374.00
Plus shipping costs $45.00
96 tests
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  • Target
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Brand
    AssayMax™
    Sample Type
    Cell Culture Cells, Plasma, Serum
    Analytical Method
    Quantitative
    Material not included
    Microplate reader capable of measuring absorbance at 405 nm. Pipettes (1-20 µL, 20-200 µL, and multiple channel). Deionized or distilled reagent grade water Incubator (37 °C)
  • Assay Time
    4 h
    Plate
    Pre-coated
    Calculation of Results
    • Calculate the mean value of the duplicate or triplicate readings for each standard and sample.
    • To generate a standard curve, plot the graph using the standard concentrations on the x-axis and the corresponding mean 450 nm absorbance (OD) on the y-axis. The best-fit line can be determined by regression analysis using log-log or four-parameter logistic curve-fit.
    • Determine the unknown sample concentration from the standard curve and multiply the value by the dilution factor.
    Restrictions
    For Research Use only
  • Handling Advice
    This product is for Research Use Only and is Not For Use In Diagnostic Procedures. Prepare all reagents (working diluent buffer, wash buffer, standard, biotinylated antibody, and SP Conjugate) as instructed, prior to running the assay. Prepare all samples prior to running the assay. The dilution factors for the samples are suggested in this insert. However, the user should determine the optimal dilution factor. Spin down the SP Conjugate vial and the biotinylated antibody vial before opening and using contents. The Stop Solution is an acidic solution. The kit should not be used beyond the expiration date. 2
    Storage
    4 °C,-20 °C
  • Target
    Alternative Name
    Nucleoside Diphosphate Kinase D (NDPKD) (NME4 ELISA Kit Abstract)
    Gene ID
    4833
    UniProt
    O00746
    Pathways
    Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process
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