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PPARD Reactivity: Human Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
Catalog No. ABIN5690761
Plus shipping costs $50.00
96 tests
Shipping to: United States
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  • Target See all PPARD ELISA Kits
    PPARD (Peroxisome Proliferator-Activated Receptor delta (PPARD))
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    Detection Method
    Method Type
    DNA-Binding ELISA
     Human PPAR-delta Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical PPAR-delta binding sequences to semi-quantitatively detect active PPAR-delta in lysates or nuclear extracts. 
    Sample Type
    Cell Lysate, Nuclear Extract
    Analytical Method
    The olionucleotide/antibody pair provided in this kit recognizes human PPAR-delta in whole lysates and nuclear extracts.
    • Specific transcription factor-DNA binding assay
    • Perfect alternative to EMSA
    • Easy to perform in an ELISA format
    • Non-radioactive assay
    • High throughput (96 well plate format)
    • Assay can be completed within 5 hours
    • 96-well Strip Microplate pre-coated with DNA probes
    • DNA Binding Buffer
    • Positive Control Sample
    • Specific Competitor DNA probe
    • Non-specific Competitor DNA probe
    • Assay Reagent
    • DTT
    • Wash Buffer
    • Primary Antibody
    • HRP-conjugated Secondary Antibody
    • TMB One-Step Substrate Reagent
    • Stop Solution
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions Absorbent paper
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Discover our best selling PPARD ELISA Kit
  • Plate
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared HRP-secondary antibody to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    For Research Use only
  • Storage
    4 °C
    Storage Comment
    Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
    Expiry Date
    6 months
  • Target See all PPARD ELISA Kits
    PPARD (Peroxisome Proliferator-Activated Receptor delta (PPARD))
    Alternative Name
    PPAR-delta (PPARD Products)
    FAAR ELISA Kit, NR1C2 ELISA Kit, NUC1 ELISA Kit, NUCI ELISA Kit, NUCII ELISA Kit, PPARB ELISA Kit, NUC-1 ELISA Kit, Nr1c2 ELISA Kit, PPAR-beta ELISA Kit, PPAR-delta ELISA Kit, PPAR[b] ELISA Kit, PPARdelta ELISA Kit, Pparb ELISA Kit, Pparb/d ELISA Kit, PPARBETA ELISA Kit, ppard ELISA Kit, pparb ELISA Kit, PPARD ELISA Kit, PPAR-D/B ELISA Kit, peroxisome proliferator activated receptor delta ELISA Kit, peroxisome proliferator activator receptor delta ELISA Kit, peroxisome proliferator activated receptor delta S homeolog ELISA Kit, peroxisome proliferator-activated receptor delta ELISA Kit, PPARD ELISA Kit, Ppard ELISA Kit, ppard.S ELISA Kit, ppard ELISA Kit
    Gene ID
    Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Monocarboxylic Acid Catabolic Process, Smooth Muscle Cell Migration, Positive Regulation of fat Cell Differentiation
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