Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

IL17 Receptor B ELISA Kit

IL17RB Reactivity: Mouse Colorimetric Sandwich ELISA 0.2-50 ng/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN625406
  • Target See all IL17 Receptor B (IL17RB) ELISA Kits
    IL17 Receptor B (IL17RB) (Interleukin 17 Receptor B (IL17RB))
    Reactivity
    • 8
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.2-50 ng/mL
    Minimum Detection Limit
    0.2 ng/mL
    Application
    ELISA
    Purpose
    Mouse IL-17 RB ELISA Kit for serum, plasma, and cell culture supernatant samples.
    Sample Type
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse Adiponectin (ACRP30), ANGPTL1, ANGPTL3, Artemin, CCL28 (MEC), CD36 (SR-B3), Chordin, CRP, E-Cadherin, Epigen, Epiregulin, Fas, Galectin-7, gp130, Granzyme B, Gremlin-1, IFN-gamma R1, IL-17B, IL-22, MIP-1beta, MMP-10, MMP-2, MMP-3, PDGF-AA, Persephin, sFRP-3, SLAM, Shh-N, TCK-1, TECK, TGF beta 1, Trance, TREML1, TWEAK, VEGF-B, VEGFR2
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling IL17RB ELISA Kit
    Top Product
    Discover our top product IL17RB ELISA Kit
  • Application Notes
    Recommended Dilution for serum and plasma samples2 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all IL17 Receptor B (IL17RB) ELISA Kits
    IL17 Receptor B (IL17RB) (Interleukin 17 Receptor B (IL17RB))
    Alternative Name
    IL-17 RB (IL17RB Products)
    Synonyms
    IL17RB ELISA Kit, CRL4 ELISA Kit, EVI27 ELISA Kit, IL17BR ELISA Kit, IL17RH1 ELISA Kit, Evi27 ELISA Kit, IL-17ER ELISA Kit, IL-17Rh1 ELISA Kit, Il17br ELISA Kit, interleukin 17 receptor B ELISA Kit, IL17RB ELISA Kit, Il17rb ELISA Kit
    Gene ID
    50905
    UniProt
    Q9JIP3
You are here:
Support