CLEC3B ELISA Kit

Catalog No. ABIN6720311

Supplier Product No.: koa0738

Product Details CLEC3B ELISA Kit

Target: CLEC3B (C-Type Lectin Domain Family 3, Member B (CLEC3B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 312 pg/mL - 20000 pg/mL

Minimum Detection Limit: 312 pg/mL

Application: ELISA

Supplier Product No.: koa0738

Supplier: Rockland

Purpose: Human Tetranectin - CLEC3B Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Sample Type: Cell Culture Supernatant, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human Tetranectin . There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Tetranectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or tissue Homogenates to each empty well. We recommend that each human Tetranectin standard solution and each sample is measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: E22-V202

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CLEC3B

Target: CLEC3B (C-Type Lectin Domain Family 3, Member B (CLEC3B))

Alternative Name: Tetranectin/CLEC3B (CLEC3B Products)

Background:

Synonyms: C type lectin domain family 3 member B, C-type lectin domain family 3 member B, Clec3b, Plasminogen kringle 4 binding protein, Plasminogen kringle 4-binding protein, TETN_HUMAN, Tetranectin (plasminogen binding protein), Tetranectin, TN, TNA

Background: Tetranectin, also called TNA, is a protein that in humans is encoded by the CLEC3B gene. It is mapped to 3p21.31. Tetranectin, a tetrameric protein isolated from human plasma, has 4 identical and noncovalently bound polypeptide chains, each of 181 amino acid residues. It has a specific binding affinity for sulfated polysaccharides and the kringle 4 of plasminogen. The plasma concentration of tetranectin is reduced in patients with various malignancies. Tetranectin is a plasminogen-binding protein that is induced during the mineralization phase of osteogenesis. Thus, tetranectin is a candidate gene for human disorders affecting bone and connective tissue.

Gene ID: 7123

NCBI Accession: NP_003269

UniProt: P05452