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MCAM ELISA Kit

MCAM Reactivity: Mouse Colorimetric Sandwich ELISA 0.18 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN6730098
  • Target See all MCAM ELISA Kits
    MCAM (Melanoma Cell Adhesion Molecule (MCAM))
    Reactivity
    • 6
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.18 ng/mL - 40 ng/mL
    Minimum Detection Limit
    0.18 ng/mL
    Application
    ELISA
    Purpose
    Mouse MCAM/CD146 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    Sample Type
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Mouse MCAM.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product MCAM ELISA Kit
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Reagent Preparation

    Recommended Dilution for serum and plasma samples2 fold

    Restrictions
    For Research Use only
  • Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all MCAM ELISA Kits
    MCAM (Melanoma Cell Adhesion Molecule (MCAM))
    Alternative Name
    MCAM (CD146 (MCAM Products)
    Synonyms
    CD146 ELISA Kit, MUC18 ELISA Kit, Muc18 ELISA Kit, 1-gicerin ELISA Kit, AV025631 ELISA Kit, CD149 ELISA Kit, s-endo ELISA Kit, s-gicerin ELISA Kit, cd146 ELISA Kit, gicerin ELISA Kit, id:ibd3512 ELISA Kit, mcam ELISA Kit, MCAM ELISA Kit, melanoma cell adhesion molecule ELISA Kit, melanoma cell adhesion molecule b ELISA Kit, MCAM ELISA Kit, Mcam ELISA Kit, mcamb ELISA Kit
    UniProt
    Q8R2Y2
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