Human Lipocalin-2/NGAL EZ Set™ ELISA Kit (DIY Antibody Pairs)

Sequence similarities: Belongs to the calycin superfamily. Lipocalin family.

Tissue Specificity: Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes. .

The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
The EZ Set™ Accessory Kit provides:
Antibody diluent buffer
ABC diluent buffer
Sample diluent buffer
TMB
TMB stop solution
5x 96 well plates, plate sealers

Protein Function: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. .

Background: Lipocalin-2(LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide(LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung.The standard product used in this kit is recombinant human NGAL, consisting of 178 amino acids with the molecular mass of 22KDa.

Synonyms: Neutrophil gelatinase-associated lipocalin,NGAL,25 kDa alpha-2-microglobulin-related subunit of MMP-9,Lipocalin-2,Oncogene 24p3,Siderocalin LCN2,p25,LCN2,HNL, NGAL,

Full Gene Name: Neutrophil gelatinase-associated lipocalin