Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (Middle Region) Peptide
CDC27
Reactivity: Human
Host: Synthetic
BP, WB
Catalog No. ABIN974728
Quick Overview for Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (Middle Region) Peptide (ABIN974728)
Target
CDC27
(Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB)
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Protein Region
- Middle Region
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Sequence
- KLKMKFPPKI PNRKTKSKTN KGGITQPNIN DSLEITKLDS SIISEGKIST
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Characteristics
- This is a synthetic peptide designed for use in combination with anti-CDC27 Antibody(ARP43603_P050),. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Purification
- Purified
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Concentration
- 1 mg/mL
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Buffer
- Final peptide concentration is 1 mg/mL in PBS.
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))
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Background
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The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: APC3, CDC27Hs, D0S1430E, D17S978E, HNUC, NUC2, ANAPC3
Protein Interaction Partner: FZR1,PIN1,ANAPC10,ANAPC2,ANAPC7,BUB1B,CASP3,CDC16,CDC20,CDC26,FZR1,HOXC10,MAD2L1,PIN1,PPP5C,RB1,SKIL,SMAD2,SMAD3,ANAPC1,ANAPC10,ANAPC11,ANAPC2,ANAPC4,ANAPC5,ANAPC7,APC2,BUB1B,CCNB1,CDC16,CDC20,CDC23,CDC26,CDH1,CDKN1A,CTNNB1,FZR1,MAD2L1,PIN1,PPP5C,PTTG1,Pp
Protein Size: 824 -
Molecular Weight
- 92 kDa
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Gene ID
- 996
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NCBI Accession
- NM_001256, NP_001247
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UniProt
- P30260
Target
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