NAMPT Protein (AA 1-491) (His tag)

Catalog No. ABIN1344091

Product Details

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 1-491

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This NAMPT protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Purpose: Nampt (Visfatin/PBEF) (human) (rec.) (His)

Cross-Reactivity: Human

Characteristics: Intracellular human Nampt (visfatin/PBEF) (aa 1-491) is fused at the C-terminus to a His-tag.

Purity: >90 % (SDS-PAGE)

Endotoxin Level: <1EU/μg purified protein (LAL test).

Biological Activity Comment: Shows adipogenic effects in stimulated differentiating 3T3-L1 cells.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in distilled water.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

References

Kover, Tong, Watkins, Clements, Stehno-Bittel, Novikova, Bittel, Kibiryeva, Stuhlsatz, Yan, Ye, Moore: "Expression and regulation of nampt in human islets." in: PLoS ONE, Vol. 8, Issue 3, pp. e58767, (2013) (PubMed).

Yonezawa, Haga, Kobayashi, Takahashi, Obara: "Visfatin is present in bovine mammary epithelial cells, lactating mammary gland and milk, and its expression is regulated by cAMP pathway." in: FEBS letters, Vol. 580, Issue 28-29, pp. 6635-43, (2006) (PubMed).

Target Details

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Alternative Name: Nampt (NAMPT Products)

Background:

Pre-B Cell Colony Enhancing Factor 1, PBEF1, Nicotinamide Phosphoribosyltransferase

Nicotinamide phosphoribosyltransferase (Nampt, pre-B cell colony-enhancing factor, PBEF, Visfatin) is an 52 kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

Molecular Weight: ~52kDa (SDS-PAGE)

UniProt: P43490