Bone Sialoprotein (BSP) (AA 17-317) protein (His-SUMO Tag)

Details for Product No. ABIN1877756
Protein Name
Protein Characteristics
AA 17-317
Origin
Human
1
1
Source
Escherichia coli (E. coli)
2
Protein Type
Recombinant
Purification tag / Conjugate
His-SUMO Tag
Application
Immunoprecipitation (IP), SDS-PAGE (SDS), ELISA, Western Blotting (WB)
Options
Sequence FSMK NLHRRVKIED SEENGVFKYR PRYYLYKHAY FYPHLKRFPV QGSSDSSEEN GDDSSEEEEE EEETSNEGEN NEESNEDEDS EAENTTLSAT TLGYGEDATP GTGYTGLAAI QLPKKAGDIT NKATKEKESD EEEEEEEEGN ENEESEAEVD ENEQGINGTS TNSTEAENGN GSSGGDNGEE GEEESVTGAN AEDTTETGRQ GKGTSKTTTS PNGGFEPTTP PQVYRTTSPP FGKTTTVEYE GEYEYTGANE YDNGYEIYES ENGEPRGDNY RAYEDEYSYF KGQGYDGYDG QNYYHHQ
Characteristics Phe17-Gln317 with Two N-terminal Tags, His-tag and SUMO-tag
Purity > 95 %
Endotoxin Level < 1.0 EU per 1 µg (determined by the LAL method)
Protein Name
Molecular Weight 90kDa
UniProt P21815
Comment

The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 °C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Buffer 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months