VEGF-A164 (AA 27-190) Protein
Origin: Mouse
Host: Yeast (Pichia pastoris)
Recombinant
> 95 % as determined by reducing SDS-PAGE.
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- Target
- VEGF-A164
- Protein Type
- Recombinant
- Protein Characteristics
- AA 27-190
- Origin
- Mouse
- Source
- Yeast (Pichia pastoris)
- Purpose
- Recombinant Mouse VEGF-A/VEGF164
- Sequence
- APTTEGEQKS HEVIKFMDVY QRSYCRPIET LVDIFQEYPD EIEYIFKPSC VPLMRCAGCC NDEALECVPT SESNITMQIM RIKPHQSQHI GEMSFLQHSR CECRPKKDRT KPENHCEPCS ERRKHLFVQD PQTCKCSCKN TDSRCKARQL ELNERTCRCD KPRR
- Characteristics
- Recombinant Mouse VEGF-A164 is produced by our Pichia pastoris expression system. The target protein is expressed with sequence (Ala27-Arg190) of Mouse VEGF-1.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- VEGF-A164
- Background
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Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult . It is a member of the PDGF family that is characterized by a cystine knot structure formed by eight conserved cysteine residues . Alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) have been identified in humans, with 120, 164 and 188 aa isoforms found in mouse . VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-/KDR) on endothelial cells .Although affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity . Human VEGF165 binds the semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2 . VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells . In adults, VEGF functions mainly in wound healing and the female reproductive cycle . Pathologically, it is involved in tumor angiogenesis and vascular leakage . Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M and TNF-α .
Synonyms: Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGFA, VEGFA164, VEGF164 - Molecular Weight
- 19.27 kDa
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