Syntrophin, gamma 2 (SNTG2) (AA 1-539) protein (His tag)

Details for Product No. ABIN3076997
Protein Name
Protein Characteristics
AA 1-539
1
Origin
Human
1
1
Source
Insect Cells
1
1
Protein Type
Recombinant
Purification tag / Conjugate
This SNTG2 protein is labelled with His tag.
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Options
Sequence MGTEGPPPPA ASRGRQGCLL VPARTKTTIA LLYDEESENA YDIRLKLTKE VLTIQKQDVV CVGGSHQGRN RRTVTLRRQP VGGLGLSIKG GSEHNVPVVI SKIFEDQAAD QTGMLFVGDA VLQVNGIHVE NATHEEVVHL LRNAGDEVTI TVEYLREAPA FLKLPLGSPG PSSDHSSGAS SPLFDSGLHL NGNSSTTAPS SPSSPIAKDP RYEKRWLDTL SVPLSMARIS RYKAGTEKLR WNAFEVLALD GVSSGILRFY TAQDGTDWLR AVSANIRELT LQNMKMANKC CSPSDQVVHM GWVNEKLQGA DSSQTFRPKF LALKGPSFYV FSTPPVSTFD WVRAERTYHL CEVLFKVHKF WLTEDCWLQA NLYLGLQDFD FEDQRPYCFS IVAGHGKSHV FNVELGSELA MWEKSFQRAT FMEVQRTGSR TYMCSWQGEM LCFTVDFALG FTCFESKTKN VLWRFKFSQL KGSSDDGKTR VKLLFQNLDT KQIETKELEF QDLRAVLHCI HSFIAAKVAS VDPGFMDSQS LARKYMYSS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Characteristics
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Human SNTG2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Purity >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterility 0.22 μm filtered
Endotoxin Level Protein is endotoxin free.
Grade Crystallography grade
Protein Name
Background Adapter protein that binds to and probably organizes the subcellular localization of a variety of proteins. May link various receptors to the actin cytoskeleton and the dystrophin glycoprotein complex (By similarity). {ECO:0000250}.
Molecular Weight 61.2 kDa Including tag.
UniProt Q9NY99
Application Notes In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
Comment

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80°C.
Expiry Date Unlimited (if stored properly)
Supplier Images
Image no. 1 for Syntrophin, gamma 2 (SNTG2) (AA 1-539) protein (His tag) (ABIN3076997) „Crystallography Grade“ protein due to multi-step, protein-specific purification process