SLC26A8 Protein (AA 1-970) (Strep Tag)

Catalog No. ABIN3117549

Product Details

Target: SLC26A8 (Solute Carrier Family 26 (Sulfate Transporter), Member 8 (SLC26A8))

Protein Type: Recombinant

Protein Characteristics: AA 1-970

Origin: Human

Source: Cell-free protein synthesis (CFPS)

Purification tag / Conjugate: This SLC26A8 protein is labelled with Strep Tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Brand: AliCE®

Sequence: MAQLERSAIS GFSSKSRRNS FAYDVKREVY NEETFQQEHK RKASSSGNMN INITTFRHHV QCRCSWHRFL RCVLTIFPFL EWMCMYRLKD WLLGDLLAGI SVGLVQVPQG LTLSLLARQL IPPLNIAYAA FCSSVIYVIF GSCHQMSIGS FFLVSALLIN VLKVSPFNNG QLVMGSFVKN EFSAPSYLMG YNKSLSVVAT TTFLTGIIQL IMGVLGLGFI ATYLPESAMS AYLAAVALHI MLSQLTFIFG IMISFHAGPI SFFYDIINYC VALPKANSTS ILVFLTVVVA LRINKCIRIS FNQYPIEFPM ELFLIIGFTV IANKISMATE TSQTLIDMIP YSFLLPVTPD FSLLPKIILQ AFSLSLVSSF LLIFLGKKIA SLHNYSVNSN QDLIAIGLCN VVSSFFRSCV FTGAIARTII QDKSGGRQQF ASLVGAGVML LLMVKMGHFF YTLPNAVLAG IILSNVIPYL ETISNLPSLW RQDQYDCALW MMTFSSSIFL GLDIGLIISV VSAFFITTVR SHRAKILLLG QIPNTNIYRS INDYREIITI PGVKIFQCCS SITFVNVYYL KHKLLKEVDM VKVPLKEEEI FSLFNSSDTN LQGGKICRCF CNCDDLEPLP RILYTERFEN KLDPEASSIN LIHCSHFESM NTSQTASEDQ VPYTVSSVSQ KNQGQQYEEV EEVWLPNNSS RNSSPGLPDV AESQGRRSLI PYSDASLLPS VHTIILDFSM VHYVDSRGLV VLRQICNAFQ NANILILIAG CHSSIVRAFE RNDFFDAGIT KTQLFLSVHD AVLFALSRKV IGSSELSIDE SETVIRETYS ETDKNDNSRY KMSSSFLGSQ KNVSPGFIKI QQPVEEESEL DLELESEQEA GLGLDLDLDR ELEPEMEPKA ETETKTQTEM EPQPETEPEM EPNPKSRPRA HTFPQQRYWP MYHPSMASTQ SQTQTRTWSV ERRRHPMDSY SPEGNSNEDV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).

Purity: > 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SLC26A8 (Solute Carrier Family 26 (Sulfate Transporter), Member 8 (SLC26A8))

Alternative Name: SLC26A8 (SLC26A8 Products)

Background: Testis anion transporter 1 (Anion exchange transporter) (Solute carrier family 26 member 8),FUNCTION: Antiporter that mediates the exchange of sulfate and oxalate against chloride ions across a membrane (PubMed:11834742, PubMed:11278976). Stimulates anion transport activity of CFTR (PubMed:22121115, PubMed:23582645). May cooperate with CFTR in the regulation of chloride and bicarbonate ions fluxes required for activation of the ADCY10/PKA pathway during sperm motility and sperm capacitation (By similarity). May play a role in sperm tail differentiation and motility and hence male fertility (By similarity). {ECO:0000250|UniProtKB:Q8R0C3, ECO:0000269|PubMed:11278976, ECO:0000269|PubMed:11834742, ECO:0000269|PubMed:22121115, ECO:0000269|PubMed:23582645}.

Molecular Weight: 109.0 kDa

UniProt: Q96RN1

Pathways: Dicarboxylic Acid Transport