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CCDC57 Protein (AA 1-1016) (Strep Tag)

CCDC57 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Catalog No. ABIN3123760
  • Target See all CCDC57 products
    CCDC57 (Coiled-Coil Domain Containing 57 (CCDC57))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-1016
    Origin
    Mouse
    Source
    • 1
    Tobacco (Nicotiana tabacum)
    Purification tag / Conjugate
    This CCDC57 protein is labelled with Strep Tag.
    Application
    ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    MLPLCSEREL NELLARKEEE WRVLQAHRAQ LQEAALQAAQ NRLEETQGKL QRLQEDFVYN LQVLEERDRE LERYDVEFTQ ARQREEAQQA EASELKIEVA KLKQDLTREA RRVGELQHQH QLMLQEHRLE LERVHSDKNS ELAHQREQNE RLEWELERKL KELDGELALQ RQELLLEFES KMQRREHEFQ LRADDMSNVV LTHELKIKLL NKELQALRDA GARAAESLQK AEAEHVELER KLQERARELQ DLEAVKDARI KGLEKKLYSA QLAKKKAEET FRRKHEELDR QAREKDTVLA AVKRAHAEEL QTLDAKVLEL QFLCETLEGQ LRRAECTRAE DAKEKNALTD KFREDAAALK AAWDAQITQM SKETVSKDFQ IHTLQEEEMK LKAQVARFQQ DIDRYKQQLS LAVERGQSLE REQVQLGLDW QRRCDDIERD QIQKSETLIE GLTKARDQVA AKLQETEKAL RQQETLLKAV SLERDQAMET LRTHGLLPGQ EAQVPPQQHE GEIRADSPST EIQRLQEQNA GLRNAVSQMR REMEMLSGHL PPAQPEECSN ADPDPKAGGD STPPDYVLTL EAEMQNLKHK LKALEEQLQS TEEPVKTSVA TADPHHGVHS SAAAADAALA DQTSTALALR KLGDRVHLLN LLVTQLKRKL RQKPRELVPV QHEVPSEVDQ VHLEVLELQK QVAELRKHLK VTPQGEPSSR EQLQRQGVAD RYPMGMEDQT ESPTFPQEGA QPPQTIYVTH LQRKLKDAAR KILSLRLERE QLLEMGNRLR AEQGHAKGKP TPCPGPPTSE PQDPQEVPER SLDRGPPLGQ LQPYSTTQDP RHTKRRCASE YAGKSQPHSA QVGSKTNTPR GHKAEMASRP AQLSQKQHRI PTETWKPVYQ KENRTPKLPQ AHEVPEESDH RTHRSSSLAS SSLQDIWRLL ELGSSPSGVP SQDNSVAECP APSRPSCFQK VNRSPVPIQK AFAVKGLKME AQPKATPPRP SKSHPAKPTN CQQQRPSRIR NYNLKD
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Characteristics
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    Expression System:

    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Endotoxin Level
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    CCDC57 (Coiled-Coil Domain Containing 57 (CCDC57))
    Alternative Name
    Ccdc57 (CCDC57 Products)
    Synonyms
    4933434G05Rik Protein, coiled-coil domain containing 57 Protein, CCDC57 Protein, Ccdc57 Protein
    Background
    Coiled-coil domain-containing protein 57,FUNCTION: Pleiotropic regulator of centriole duplication, mitosis, and ciliogenesis (PubMed:32402286). Critical interface between centrosome and microtubule-mediated cellular processes. Centriole duplication protein required for recruitment of CEP63, CEP152, and PLK4 to the centrosome. Independent of its centrosomal targeting, localizes to and interacts with microtubules and regulates microtubule nucleation, stability, and mitotic progression (By similarity). {ECO:0000250|UniProtKB:Q2TAC2, ECO:0000269|PubMed:32402286}.
    Molecular Weight
    116.2 kDa
    UniProt
    Q6PHN1
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