MOCS3 Protein (AA 1-460) (His tag)

Catalog No. ABIN3130852

Product Details

Target: MOCS3 (Molybdenum Cofactor Synthesis 3 (MOCS3))

Protein Type: Recombinant

Protein Characteristics: AA 1-460

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This MOCS3 protein is labelled with His tag.

Application: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MAAPEDVAAL QAEITRREEE LASLKRRLAA ALTAEPEPER PLRVPPPPLA PRAALSRDEI LRYSRQLLLP ELGVRGQLRL AAAAVLVVGC GGLGCPLAQY LAAAGVGRLG LVDHDVVETS NLARQVLHGE AQAGESKARS AAAALRRLNS AVECVAYPRA LAEDWALDLV RGYDVVADCC DNVPTRYLVN DACVLAGRPL VSASALRFEG QMTVYHHDGG PCYRCVFPRP PPPETVTNCA DGGVLGAVPG VLGCAQALEV LKIAAGLGSS YSGSMLLFDG LGGHFRRIRL RRRRPDCVVC GQQPTVTRLQ DYEAFCGSSA TDKCRALKLL CPEERISVTD YKRLLDSGAP HVLLDVRPQV EVDICRLPHS LHIPLSQLER RDADSLKLLG AALRKGKQES QEGVALPVYV ICKLGNDSQK AVKVLQSLTA VPELDSLTVQ DIVGGLMAWA AKIDGTFPQY
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Mocs3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: MOCS3 (Molybdenum Cofactor Synthesis 3 (MOCS3))

Alternative Name: Mocs3 (MOCS3 Products)

Synonyms: MOCS3 Protein, DDBDRAFT_0189692 Protein, DDBDRAFT_0267172 Protein, DDB_0189692 Protein, DDB_0267172 Protein, UBA4 Protein, 1700020H17Rik Protein, Uba4 Protein, zgc:55696 Protein, uba4 Protein, molybdenum cofactor synthesis 3 Protein, molybdenum cofactor synthesis 3 L homeolog Protein, MOCS3 Protein, mocs3 Protein, Mocs3 Protein, mocs3.L Protein

Background: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor, NFS1 probably acting as a sulfur donor for thiocarboxylation reactions. {ECO:0000255|HAMAP-Rule:MF_03049}.

Molecular Weight: 50.3 kDa Including tag.

UniProt: A2BDX3