Phospholipase C beta 2 Protein (AA 1-1181) (His tag)

Catalog No. ABIN3130884

Product Details

Target: Phospholipase C beta 2 (PLCb2) (phospholipase C beta 2 (PLCb2))

Protein Type: Recombinant

Protein Characteristics: AA 1-1181

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This Phospholipase C beta 2 protein is labelled with His tag.

Application: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MSLLNPVLLP PNVKAYLSQG ERFIKWDDET SIASPVILRV DPKGYYLYWT YQNQEMEFLD VTSIRDTRFG KFAKIPKSQK LREVFNMDFP DNHFLLKTLT VVSGPDMVDL TFYNFVSYKE NVGKDWAEDV LALAKHPMTV NAPRSTFLDK ILVKLKMQLN PEGKIPVKNF FQMFPADRKR VEAALGACHL AKGKNDAINP EDFPESVYKS FLMSLCPRPE IDEIFTSYHS KAKPYMTKEH LTKFINQKQR DPRLNSLLFP PARPEQVQVL IDKYEPSGIN VQRGQLSPEG MVWFLCGPEN SVLAHDTLLI HQDMTQPLNH YFINSSHNTY LTAGQFSGLS SAEMYRQVLL SGCRCVELDC WKGKPPDEEP IITHGFTMTT DILFKEAIEA IAESAFKTSP YPVILSFENH VDSPRQQAKM AEYCRSMFGE TLLTDPLENF PLKPGIPLPS PEDLRGKILI KNKKNQFSGP ASPSKKPGGV AEGSLPSSVP VEEDTGWTAE DRTEVEEEEV VEEEEEEESG NLDEEEIKKM QSDEGTAGLE VTAYEEMSSL VNYIQPTKFI SFEFSAQKNR SYVVSSFTEL KAYELLSKAS MQFVDYNKRQ MSRVYPKGTR MDSSNYMPQM FWNAGCQMVA LNFQTMDLPM QQNMALFEFN GQSGYLLKHE FMRRLDKQFN PFSVDRIDVV VATTLSITII SGQFLSERSV RTYVEVELFG LPGDPKRRYR TKLSPTANSI NPVWKEEPFI FEKILMPELA SLRIAVMEEG SKFLGHRIIP INALHSGYHH LCLRSESNMA LTMPALFVFL EMKDYIPDTW ADLTVALANP IKYFNAQDKK SVKLKGVTGS LPEKLFSGTP VASQSNGAPV SAGNGSTAPG TKATGEEATK EVTEPQTASL EELRELKGVV KLQRRHEKEL RELERRGARR WEELLQRGAA QLAELQTQAA GCKLRPGKGS RKKRTLPCEE TVVAPSEPHD RADPRVQELK DRLEQELQQQ GEEQYRSVLK RKEQHVTEQI AKMMELAREK QAAELKTFKE TSETDTKEMK KKLEAKRLER IQAMTKVTTD KVAQERLKRE INNSHIQEVV QAVKQMTETL ERHQEKLEER QTACLEQIQA MEKQFQEKAL AEYEAKMKGL EAEVKESVRA YFKDCFPTEA EDKPERSCEA SEESCPQEPL VSKADTQESR L
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Plcb2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Phospholipase C beta 2 (PLCb2) (phospholipase C beta 2 (PLCb2))

Alternative Name: Plcb2 (PLCb2 Products)

Synonyms: AI550384 Protein, B230205M18Rik Protein, B230399N12 Protein, phospholipase C, beta 2 Protein, phospholipase C beta 2 Protein, Plcb2 Protein, PLCB2 Protein

Background: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This protein may be involved in the transduction of bitter taste stimuli (By similarity). {ECO:0000250}.

Molecular Weight: 135.5 kDa Including tag.

UniProt: A3KGF7

Pathways: WNT Signaling, Thyroid Hormone Synthesis, CXCR4-mediated Signaling Events, G-protein mediated Events, Thromboxane A2 Receptor Signaling