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THEMIS Protein (AA 1-636) (His tag)

Crystallography grade THEMIS Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3136150
$16,803.00
Plus shipping costs $45.00 and $20.00 dry ice
Shipping to: United States
Will be delivered in 60 Business Days
  • Target See all THEMIS Proteins
    THEMIS (Thymocyte Selection Associated (THEMIS))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-636
    Origin
    Mouse
    Source
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This THEMIS protein is labelled with His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    MALSLEEFVY SLDLRTLPRV LEIQSGIYFE GSVYEMFGNE CCLSTGEVIK ITGLKIKKMM AEICEGAIGG CESQKPFELP MNFPGLFKVM ADKTPYLSIE EITRTVNIGP SRLGHPCFYH LKDIKLENLI IKQGEPIRFN SVEEINGETL VNCGVVRNHQ SHSFTLPLSQ EGEFYECEDE HIYTLKEIVE WKIPKNRTRT VKLTDFSNKW DSTNPFPEDF YGTLILKPVY EIQGVLKFQK DIVRILPSLD VEVKDITDSY DANWFLQLLS TDDLFEMTSK EFPVVAEVVE ISQGNHLPQS ILQREKTIVI HKKYQASRIL ASEIRSNFPK RHFLIPISYK GKFKRRPREF PTAYDLQIAK SRKETLHVVA TKAFHTLHKE LSPVSVGDQF LVHHSETTEV VFEGTRKVNV LTCEKVLNKT REDAQLPLYM EGGFVEVIHD KKQYQISELC TQFCWPFNVK VAVRDLSIKD DILAATPGLQ LEEDITDSYL LISDFANPEE CWEIPMSRLN MTVRLVNGSS LPADAGLLQV RSFVEEITEE QYYMMRRYES SLSHPPPRPP KHPSAEEMKL TLLSLAEERT INLPKSLKSH HVDRPKKLPS DESGQDSRAP VGFQNDVADV ERQKSKHGPL QPQAPL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Themis Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    THEMIS (Thymocyte Selection Associated (THEMIS))
    Alternative Name
    Themis (THEMIS Products)
    Synonyms
    TSEPA Protein, C6orf190 Protein, C6orf207 Protein, GASP Protein, SPOT Protein, bA325O24.3 Protein, bA325O24.4 Protein, A430108D01 Protein, BB160712 Protein, E430004N04Rik Protein, Gasp Protein, Tsepa Protein, thylex Protein, C9H6ORF190 Protein, im:7138580 Protein, tsepa Protein, thymocyte selection associated Protein, THEMIS Protein, Themis Protein, themis Protein
    Background
    Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk. {ECO:0000269|PubMed:19597497, ECO:0000269|PubMed:19597498, ECO:0000269|PubMed:19597499, ECO:0000269|PubMed:19620281, ECO:0000269|PubMed:19805304}.
    Molecular Weight
    73.7 kDa Including tag.
    UniProt
    Q8BGW0
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