Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

OSBPL8 Protein (AA 1-889) (rho-1D4 tag)

Crystallography grade OSBPL8 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3131063
$26,631.34
Plus shipping costs $45.00 and $20.00 dry ice
Shipping to: United States
Delivery in 60 to 65 Business Days
  • Target See all OSBPL8 Proteins
    OSBPL8 (Oxysterol Binding Protein-Like 8 (OSBPL8))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-889
    Origin
    Mouse
    Source
    • 2
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This OSBPL8 protein is labelled with rho-1D4 tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    MEAALADGEP DRSSLLGDSK DVLGPSTVVA NSDEPQHLTP GKMSQRQGRD ANPTPTRDLP QPSLSPASLH SQGFERGKED ISQNKDDSSL SMSKSKSESK LYNGSEKDSS TSSKLTKKES LKVQKKNYRE EKKRATKELL STITDPSVIV MADWLKIRGT LKSWTKLWCV LKPGVLLIYK TQKNGQWVGT VLLNACEIIE RPSKKDGFCF KLFHPLEQSI WAVKGPKGEA VGSITQPLPS SYLIIRATSE SDGRCWMDAL ELALKCSSLL KRTMVREGKE HDLSISSDST HVTLYGLLRA NNLHSGDNFQ LNDSEIERQH FKDQDLYSDK SDKENDPEHD ESDNEVLGKS EESDTDTSER QDDSYIDPEP VEPLKETTYM EQSHEELGEA GEASQTETVS EENKSLIWTL LKQVRPGMDL SRVVLPTFIL EPRSFLDKLS DYYYHADFLS EAALEENPYF RLKKVVKWYL SGFYKKPKGL KKPYNPILGE TFRCLWIHPR TNSKTFYIAE QVSHHPPISA FYVSNRKDGF CLSGSILAKS KFYGNSLSAI LEGEARLTFL NRGEDYVMTM PYAHCKGILY GTMTLELGGT VNITCQKTGY SAILEFKLKP FLGSSDYVNQ ISGKLKLGKE VLATLEGHWD SEVFINDKKT DNSEIFWNPT PDIKQWRLIR HTVKFEEQDD FESEKLWQRV TKAINAKDQT EATQEKYVLE EAQRQAARDR KTKTQEWVCK LFELDPLTGE WHYKFSDTRP WDPLNDMIQF EKDGVIQTKV KHRTPMVSVP KMKHKPTRQQ KKVVKGYSSP EPDIQDSSGS EAQSVKPSTR RKKGIDLGDI QSSIESIKQT QEEIKRNIMA LRNHLLSSTP ATDYFLQQKD YFVIFLLILL QVIINFIFK
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Osbpl8 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin-free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    OSBPL8 (Oxysterol Binding Protein-Like 8 (OSBPL8))
    Alternative Name
    Osbpl8 (OSBPL8 Products)
    Synonyms
    RGD1561474 Protein, DKFZp469P0923 Protein, MST120 Protein, MSTP120 Protein, ORP8 Protein, OSBP10 Protein, AA536976 Protein, AA536995 Protein, C730029P18Rik Protein, D330025H14Rik Protein, ORP-8 Protein, oxysterol binding protein-like 8 Protein, oxysterol binding protein like 8 Protein, oxysterol binding protein like 8 L homeolog Protein, oxysterol-binding protein-related protein 8 Protein, Osbpl8 Protein, OSBPL8 Protein, osbpl8.L Protein, osbpl8 Protein, LOC100175952 Protein
    Background
    Lipid transporter involved in lipid countertransport between the endoplasmic reticulum and the plasma membrane: specifically exchanges phosphatidylserine with phosphatidylinositol 4-phosphate (PI4P), delivering phosphatidylserine to the plasma membrane in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds phosphatidylserine and PI4P in a mutually exclusive manner. Binds oxysterol, 25-hydroxycholesterol and cholesterol. {ECO:0000250|UniProtKB:Q9BZF1}.
    Molecular Weight
    102.4 kDa Including tag.
    UniProt
    B9EJ86
You are here:
Support