Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) (Active) Protein

Details for Product No. ABIN3170542
Protein Name
Origin
Human
17
4
1
1
Source
Escherichia coli (E. coli)
13
6
2
2
Protein Type
Recombinant
Biological Activity
Active
Options
Characteristics ~10,000 units/mg. One unit cleaves 1nmole of the caspase substrate VDVAD-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
Protein Name
Background Caspase-2 (also known as Ich-1, Nedd-2) is a member of the interleukin-1 converting enzyme (ICE) family of cysteine proteases. Similar as other caspases, caspase-2 also exists in cells as an inactive proenzyme. During apoptosis, procaspase-2 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases. The processed form of caspase-2 consists of large (19 kDa) and small (12 kDa) subunits, which associate to form the active enzyme. The active recombinant human caspase-2 was expressed in E. coli. The expressed caspase-2 spontaneously undergoes auto-processing to yield the subunits characteristic of the native enzyme.
Pathways Apoptosis, Caspase Cascade in Apoptosis, Neurotrophin Signaling Pathway
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute with PBS to 1 unit/µl.
Storage -80 °C
Supplier Images
Image no. 1 for Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) (Active) protein (ABIN3170542) Active human caspase was expressed in E. coli and purified. The activity of recombina...