DDK-1 protein (His tag,Biotin)

Catalog No. ABIN7319922

Product Details

Target: DDK-1

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purification tag / Conjugate: His tag,Biotin

Purpose: Recombinant Human Dickkopf-Related Protein 1/DKK-1 (C-Avi-6His) Biotinylated

Sequence: Thr32-His266

Characteristics: Biotinylated Recombinant Human Dickkopf-related protein 1 is produced by our Mammalian expression system and the target gene encoding Thr32-His266 is expressed with a Avi, 6His tag at the C-terminus.

Purity: >95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Comment:

35-50 kDa

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: DDK-1

Background:

Background: Dickkopf-related protein 1(DKK-1), is a member of the dickkopf family. DKK1 secreted proteins with two cysteine-rich domains separated by a linker region. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

Synonym: Dickkopf-related protein 1, Dickkopf-1, Dkk-1

Molecular Weight: 28.4 kDa

UniProt: O94907