SFI1 Protein (AA 1-1242) (His tag)

Catalog No. ABIN7555423

Product Details

Target: SFI1 (Sfi1 Homolog, Spindle Assembly Associated (SFI1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1242

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This SFI1 protein is labelled with His tag.

Purpose: Custom-made recombinant SFI1 Protein expressed in mammalian cells.

Sequence: MKNLLTEKCI SSHNFHQKVI KQRMEKKVDS RYFKDGAVKK PYSAKTLSNK KSSASFGIRR ELPSTSHLVQ YRGTHTCTRQ GRLRELRIRC VARKFLYLWI RMTFGRVFPS KARFYYEQRL LRKVFEEWKE EWWVFQHEWK LCVRADCHYR YYLYNLMFQT WKTYVRQQQE MRNKYIRAEV HDAKQKMRQA WKSWLIYVVV RRTKLQMQTT ALEFRQRIIL RVWWSTWRQR LGQVRVSRAL HASALKHRAL SLQVQAWSQW REQLLYVQKE KQKVVSAVKH HQHWQKRRFL KAWLEYLQVR RVKRQQNEMA ERFHHVTVLQ IYFCDWQQAW ERRESLYAHH AQVEKLARKM ALRRAFTHWK HYMLLCAEEA AQFEMAEEHH RHSQLYFCFR ALKDNVTHAH LQQIRRNLAH QQHGVTLLHR FWNLWRSQIE QKKERELLPL LHAAWDHYRI ALLCKCIELW LQYTQKRRYK QLLQARADGH FQQRALPAAF HTWNRLWRWR HQENVLSARA TRFHRETLEK QVFSLWRQKM FQHRENRLAE RMAILHAERQ LLYRSWFMWH QQAAARHQEQ EWQTVACAHH RHGRLKKAFC LWRESAQGLR TERTGRVRAA EFHMAQLLRW AWSQWRECLA LRGAERQKLM RADLHHQHSV LHRALQAWVT YQGRVRSILR EVAARESQHN RQLLRGALRR WKENTMARVD EAKKTFQAST HYRRTICSKV LVQWREAVSV QMYYRQQEDC AIWEAQKVLD RGCLRTWFQR WWDCSRRSAQ QRLQLERAVQ HHHRQLLLEG LARWKTHHLQ CVRKRLLHRQ STQLLAQRLS RTCFRQWRQQ LAARRQEQRA TVRALWFWAF SLQAKVWATW LAFVLERRRK KARLQWALQA YQGQLLQEGA TRLLRFAASM KASRQQLQAQ QQVQAAHSLH RAVRRCATLW KQKVLGRGGK PQPLAAIAPS RKVTFEGPLL NRIAAGAGDG TLETKRPQAS RPLGALGRLA AEEPHALELN TAHSARKQPR RPHFLLEPAQ SQRPQKPQEH GLGMAQPAAP SLTRPFLAEA PTALVPHSPL PGALSSAPGP KQPPTASTGP ELLLLPLSSF MPCGAAAPAR VSAQRATPRD KPPVPSSLAS VPDPHLLLPG DFSATRAGPG LSTAGSLDLE AELEEIQQQL LHYQTTKQNL WSCRRQASSL RRWLELNREE PGPEDQEVEQ QVQKELEQVE MQIQLLAEEL QAQRQPIGAC VARIQALRQA LC Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SFI1 (Sfi1 Homolog, Spindle Assembly Associated (SFI1))

Alternative Name: SFI1 (SFI1 Products)

Background: Protein SFI1 homolog (hSFI1),FUNCTION: Plays a role in the dynamic structure of centrosome-associated contractile fibers via its interaction with CETN2. {ECO:0000269|PubMed:16956364}.

Molecular Weight: 147.7 kDa

UniProt: A8K8P3

AlphaFold: A8K8P3

Pathways: M Phase