ATP8A2 Protein (AA 1-1148) (His tag)
custom-made
ATP8A2
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all ATP8A2 Proteins
- ATP8A2 (ATPase, Class I, Type 8A, Member 2 (ATP8A2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1148
- Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ATP8A2 protein is labelled with His tag.
- Purpose
- Custom-made recombinant Atp8a2 Protein expressed in mammalian cells.
- Sequence
- MSRATSVGDQ LEAPARIIYL NQSHLNKFCD NRISTAKYSV LTFLPRFLYE QIRRAANAFF LFIALLQQIP DVSPTGRYTT LVPLVIILTI AGIKEIIEDF KRHKADNAVN KKKTIVLRNG MWHTIMWKEV AVGDIVKVLN GQYLPADMVL FSSSEPQGMC YVETANLDGE TNLKIRQGLS HTTDMQTRDV LMKLSGRIEC EGPNRHLYDF TGNLHLDGKS SVALGPDQIL LRGTQLRNTQ WVFGVVVYTG HDSKLMQNST KAPLKRSNVE KVTNVQILVL FGILLVMALV SSVGALFWNG SHGGKSWYIK KMDTNSDNFG YNLLTFIILY NNLIPISLLV TLEVVKYTQA LFINWDMDMY YIENDTPAMA RTSNLNEELG QVKYLFSDKT GTLTCNIMNF KKCSIAGVTY GHFPELAREQ SSDDFCRMTS CTNDSCDFND PRLLKNIEDQ HPTAPCIQEF LTLLAVCHTV VPEKDGDEII YQASSPDEAA LVKGAKKLGF VFTGRTPYSV IIEAMGQEQT FGILNVLEFS SDRKRMSVIV RLPSGQLRLY CKGADNVIFE RLSKDSKYME ETLCHLEYFA TEGLRTLCVA YADLSENEYE EWLKVYQEAS IILKDRAQRL EECYEIIEKN LLLLGATAIE DRLQAGVPET IATLLKAEIK IWVLTGDKQE TAINIGYSCR LVSQNMALIL LKEDSLDATR AAITQHCTDL GNLLGKENDV ALIIDGHTLK YALSFEVRRS FLDLALSCKA VICCRVSPLQ KSEIVDVVKK RVKAITLAIG DGANDVGMIQ TAHVGVGISG NEGMQATNNS DYAIAQFSYL EKLLLVHGAW SYNRVTKCIL YCFYKNVVLY IIELWFAFVN GFSGQILFER WCIGLYNVIF TALPPFTLGI FERSCTQESM LRFPQLYRIT QNAEGFNTKV FWGHCINALV HSLILFWVPM KALEHDTPVT SGHATDYLFV GNIVYTYVVV TVCLKAGLET TAWTKFSHLA VWGSMLIWLV FFGVYSTIWP TIPIAPDMKG QATMVLSSAY FWLGLFLVPT ACLIEDVAWR AAKHTCKKTL LEEVQELETK SRVMGKAMLR DSNGKRMNER DRLIKRLSRK TPPTLFRTGS IQQCVSHGYA FSQEEHGAVT QEEIVRAYDT TKENSRKK Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- ATP8A2 (ATPase, Class I, Type 8A, Member 2 (ATP8A2))
- Alternative Name
- Atp8a2 (ATP8A2 Products)
- Background
- Phospholipid-transporting ATPase IB (EC 7.6.2.1) (ATPase class I type 8A member 2) (P4-ATPase flippase complex alpha subunit ATP8A2),FUNCTION: Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids (PubMed:22912588). Able to translocate phosphatidylserine, but not phosphatidylcholine (By similarity). Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. Reconstituted to liposomes, the ATP8A2:TMEM30A flippase complex predominantly transports phosphatidylserine (PS) and to a lesser extent phosphatidylethanolamine (PE) (PubMed:22912588). Phospholipid translocation is not associated with a countertransport of an inorganic ion or other charged substrate from the cytoplasmic side toward the exoplasm in connection with the phosphorylation from ATP (By similarity). ATP8A2:TMEM30A may be involved in regulation of neurite outgrowth (PubMed:22641037). Proposed to function in the generation and maintenance of phospholipid asymmetry in photoreceptor disk membranes and neuronal axon membranes. May be involved in vesicle trafficking in neuronal cells. Required for normal visual and auditory function, involved in photoreceptor and inner ear spiral ganglion cell survival (PubMed:24413176). {ECO:0000250|UniProtKB:C7EXK4, ECO:0000250|UniProtKB:Q9NTI2, ECO:0000269|PubMed:22641037, ECO:0000269|PubMed:22912588, ECO:0000269|PubMed:24413176}.
- Molecular Weight
- 129.4 kDa
- UniProt
- P98200
- AlphaFold
- P98200
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