DLC1 Protein (AA 1-1092) (His tag)
custom-made
DLC1
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all DLC1 products
- DLC1 (Deleted in Liver Cancer 1 (DLC1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1092
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This DLC1 protein is labelled with His tag.
- Purpose
- Custom-made recombinant Dlc1 Protein expressed in mammalian cells.
- Sequence
- MCRDEPDTMI LTQIEAKEAC DWLRVTGFPQ YAQLYEDLLF PVDIALVKRE HDFLDRDAIE ALCRRLNTLN KCAVMKLEIS PHRKRSEDSD EDEPCAISGK WTFQRDSKRW SRLEEFDVFF PKQDPIPGSP DNSRLQSATS HESMLTDLSE HQEVASVRSL SSTSSSVPTH AAHSGDATTP RTNSVISVCS SGHFVGNDDS FSSLPSPKEL SSFSFSMKGH HEKNTKSKTR SLLKRMESLK LKGSHHSKHK APSKLGLIIS APILQEGMDE AKLKQLNCVE ISALNGNHIN VPMVRKRSVS NSTQTSSSSS QSETSSAVST PSPVTRTRSL STCNKRVGMY LEGFDPFSQS TLNNVTEQNY KNRESYPEDT VFYIPEDHKP GTFPKALSHG SFCPSGNSSV NWRTGSFHGP GHLSLRRENS HDSPKELKRR NSSSSLSSRL SIYDNVPGSI LYSSSGELAD LENEDIFPEL DDILYHVKGM QRIVNQWSEK FSDEGDSDSA LDSVSPCPSS PKQIHLDVDH DRRTPSDLDS TGNSLNEPEE PTDIPERRDS GVGASLTRCN RHRLRWHSFQ SSHRPSLNSV SLQINCQSVA QMNLLQKYSL LKLTALLEKY TPSNKHGFSW AVPKFMKRIK VPDYKDRSVF GVPLTVNVQR SGQPLPQSIQ QAMRYLRNHC LDQVGLFRKS GVKSRIQALR QMNESAEDNV NYEGQSAYDV ADMLKQYFRD LPEPLMTNKL SETFLQIYQY VPKDQRLQAI KAAIMLLPDE NREVLQTLLY FLSDVTAAVK ENQMTPTNLA VCLAPSLFHL NTLKRENSSP RVMQRKQSLG KPDQKDLNEN LAATQGLAHM IAECKKLFQV PEEMSRCRNS YTEQELKPLT LEALGHLNSD QPADYRHFLQ DCVDGLFKEV KEKFKGWVSY PTSEQADLSY KKVSEGPPLR LWRSTIEVPA APEEILKRLL KEQHLWDVDL LDSKVIEILD SQTEIYQYVQ NSMAPHPARD YVVLRTWRTN LPRGACALLL TSVDHDRAPV AGVRVNVLLS RYLIEPCGSG KSKLTYMCRA DLRGHMPEWY SKSFGHLCAA EVVKIRDSFS NQNTESKDTR SR Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Deleted in Liver Cancer 1 (DLC1) (AA 1-1528) protein (His tag)
custom-made DLC1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- DLC1 (Deleted in Liver Cancer 1 (DLC1))
- Alternative Name
- Dlc1 (DLC1 Products)
- Background
- Rho GTPase-activating protein 7 (Deleted in liver cancer 1 protein homolog) (DLC-1) (Rho-type GTPase-activating protein 7) (START domain-containing protein 12) (StARD12) (StAR-related lipid transfer protein 12),FUNCTION: Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality (By similarity). Required for growth factor-induced epithelial cell migration, in resting cells, interacts with TNS3 while PTEN interacts with the p85 regulatory subunit of the PI3K kinase complex but growth factor stimulation induces phosphorylation of TNS3 and PTEN, causing them to change their binding preference so that PTEN interacts with DLC1 and TNS3 interacts with p85 (By similarity). The PTEN-DLC1 complex translocates to the posterior of migrating cells to activate RHOA while the TNS3-p85 complex translocates to the leading edge of migrating cells to promote RAC1 activation (By similarity). {ECO:0000250|UniProtKB:Q96QB1}.
- Molecular Weight
- 123.4 kDa
- UniProt
- Q9R0Z9
- AlphaFold
- Q9R0Z9
- Pathways
- Tube Formation, Positive Regulation of Endopeptidase Activity
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