Bacillus anthracis Protein

Catalog No. ABIN935200

Recombinant Bacillus anthracis protein expressed in Escherichia coli (E. coli).

Quick Overview for Bacillus anthracis Protein (ABIN935200)

Target: Bacillus anthracis

Protein Type: Recombinant

Origin: Bacillus anthracis

Source: Escherichia coli (E. coli)

Product Details

Characteristics: Purified recombinant Bacillus Anthracis (Anthrax) protein (Protective Antigen)

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from 10 mM BIS-Tris propane, pH 8.5, with 1.25 % trehalose.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C

Storage Comment: Store at 4 °C.

Target Details

Target: Bacillus anthracis

Target Type: Bacteria

Background: PA63 is the carboxyl-terminal 63- kDa fragment obtained by the proteolytic cleavage of the receptor-bound protective antigen (PA), one of the three proteins that comprise anthrax toxin. Unlike native PA, PA 63 oligomerizes to form a ring-shaped heptamer that binds up to three copies of EF and/or LF competitively and with high affinity (kDa ~1 nM). Whereas native PA persists on the cell surface, the heptamer is endocytosed, presumably because oligomerization aggregates anthrax toxin receptor. The endocytosed toxic complexes are trafficked to endosomal compartments where the low pH causes the PA63 heptamer to insert into the membrane and form a water-filled channel.
Alternative Names: Bacillus Anthracis Protein, Bacillus anthracis Protective Nicked Antigen, Anthrax Protein, Recombinant B Anthracis

Molecular Weight: 63 kDa