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Oxygen transfer studies found that 18O from [carboxyl-18O] phosphopantothenate is incorporated into AMP (show APRT Proteins) or CMP (show MATN1 Proteins) produced during PPCS catalysis, consistent with the formation of a phosphopantothenoyl cytidylate or phosphopantothenoyl adenylate intermediate
Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 126.96.36.199), one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine (Daugherty et al., 2002
, 4'-phosphopantothenoylcysteine synthetase
, phosphopantothenoylcysteine synthetase
, PPC synthetase