Recombinant Alpaca anti-Rabbit IgG Antibody (Alexa Fluor 647)

Catalog No. ABIN7272930

Product Details

Target: IgG

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Rabbit

Host: Alpaca

Expression System: E.coli

Clonality: Monoclonal

Conjugate: Alexa Fluor 647

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Rabbit IgG, does not cross-react with mouse, guinea pig or chicken immunoglobulins

Purification: Produced in: E.coli

Immunogen: This new FluoTag-X2 anti-Rabbit IgG is a species-specific FluoTag directed against IgGs from Rabbit. Especially useful for One-step immunofluorescence (IF) and Multiplexing applications.

Clone: 10E10

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Application Details

Application Notes: 1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM

Buffer: The single sdAb clone was lyophilized from PBS pH 7.4.

Storage: -20 °C/-80 °C

Storage Comment: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Like many other mammals, the immune repertoire of rabbits consists of several immunoglobulin (Ig) isotypes (IgA, IgG, IgE, and IgM). However, compared to other species, rabbits possess only one type of IgG carrying either a lambda or kappa light chain. Historically, rabbit antibodies have been a rich source of polyclonal antibodies, which were employed in various fields of life sciences. They are used either directly from animal sera, or as affinity-purified reagents. In addition, advances in antibody engineering over the last decade enabled the generation of monoclonal rabbit antibodies. Independent of its clonality and irrespectively of its VH domain, our alpaca-derived single domain antibody (sdAb, or nanobody) binds the constant domain (Fc) of any rabbit IgG, specifically and strongly, which makes this secondary tool ideal for demanding imaging applications.