CD235a/GYPA antibody (APC)

Catalog No. ABIN1112095

Product Details anti-CD235a/GYPA Antibody (APC)

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD235a/GYPA antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Characteristics: Monoclonal Mouse Anti-Human CD235a APC is recommended for use in flow cytometry.

Clone: HI264

Isotype: IgG2a

Application Details

Application Notes: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

Comment:

Allophycocyanin (Febico, Far East Bio-Tech Co.) Anti-CD235a HI264 was included in the Seventh International Workshops on Human Leucocyte Differentiation Antigens.

Sample Collection: 1. Transfer 5 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 95 µl of PBS (It better that it containing 2% bovine serum albumin) without Fixative mix gently with a vortex mixer. 3. Add 20 µl of CD235a APC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 4. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 5. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 6. Add 0, 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 7. Analyse on a flow cytometer, and the readings of the cytometer are recorded, bearing in the mind that the FSC and SSC light dispersion parameters must be on a logarithmic scale.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Preservative: Sodium azide

Precaution of Use: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Storage: 4 °C

Target Details for CD235a/GYPA

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Alternative Name: CD235a (GYPA Products)

Background: The antibody reacts with a sialoglycoprotein present on human red blood cells and embryoid precursors. Mature, non-nucleated red cells are glycophorin A positive whilst being CD45 and CD71 negative.

Pathways: Maintenance of Protein Location